A method for N-terminal de novo sequence analysis of proteins by matrix-assisted laser desorption/ionization mass spectrometry

被引：32

作者：

Kuyama, Hiroki ^{[1
]}

Sonomura, Kazuhiro ^{[2
]}

Nishimura, Osamu ^{[1
,2
]}

Tsunasawa, Susumu ^{[1
]}

机构：

[1] Osaka Univ, Inst Prot Res, Suita, Osaka 5650871, Japan

[2] Shimadzu Co Ltd, Life Sci Lab, Kyoto 6048511, Japan

来源：

ANALYTICAL BIOCHEMISTRY | 2008年 / 380卷 / 02期

关键词：

N-terminal sequence analysis; mass spectrometry; N-terminal modification; succinimidyloxycarbonylmethyl tris(2,4,6trimethoxyphenyl)phosphonium bromide;

D O I：

10.1016/j.ab.2008.05.053

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A novel method for isolation and de novo sequencing of N-terminal peptides from proteins is described. The method presented here combines selective chemical tagging using succinimidyloxycarbonylmethyl tris(2,4,6-trimethoxyphenyl)phosphonium bromide (TMPP-Ac-OSu) at the N-alpha-amino group of peptides after digestion by metalloendopeptidase (from Grfola frondosa) and selective capture procedures using p-phenylenediisothiocyanate resin, by which the IN-terminal peptide can be isolated, whether or not it is N-terminally blocked. The isolated N-terminal peptide modified N-terminally with TMPP--Ac-OSu reagent produces a simple fragmentation pattern under tandem mass spectrometric analysis to significantly facilitate sequencing. (c) 2008 Elsevier Inc. All rights reserved.

引用

页码：291 / 296

页数：6

共 27 条

[1] A METHOD FOR SELECTIVE ISOLATION OF THE AMINO-TERMINAL PEPTIDE FROM ALPHA-AMINO-BLOCKED PROTEINS [J].

AKIYAMA, TH ;

SASAGAWA, T ;

SUZUKI, M ;

TITANI, K .

ANALYTICAL BIOCHEMISTRY, 1994, 222 (01) :210-216

[2] The transcriptional landscape of the mammalian genome [J].

Carninci, P ;

Kasukawa, T ;

Katayama, S ;

Gough, J ;

Frith, MC ;

Maeda, N ;

Oyama, R ;

Ravasi, T ;

Lenhard, B ;

Wells, C ;

Kodzius, R ;

Shimokawa, K ;

Bajic, VB ;

Brenner, SE ;

Batalov, S ;

Forrest, ARR ;

Zavolan, M ;

Davis, MJ ;

Wilming, LG ;

Aidinis, V ;

Allen, JE ;

Ambesi-Impiombato, X ;

Apweiler, R ;

Aturaliya, RN ;

Bailey, TL ;

Bansal, M ;

Baxter, L ;

Beisel, KW ;

Bersano, T ;

Bono, H ;

Chalk, AM ;

Chiu, KP ;

Choudhary, V ;

Christoffels, A ;

Clutterbuck, DR ;

Crowe, ML ;

Dalla, E ;

Dalrymple, BP ;

de Bono, B ;

Della Gatta, G ;

di Bernardo, D ;

Down, T ;

Engstrom, P ;

Fagiolini, M ;

Faulkner, G ;

Fletcher, CF ;

Fukushima, T ;

Furuno, M ;

Futaki, S ;

Gariboldi, M .

SCIENCE, 2005, 309 (5740) :1559-1563

[3] Mass spectrometry: Bottom-up or top-down? [J].

Chait, Brian T. .

SCIENCE, 2006, 314 (5796) :65-66

[4] A method for the isolation of blocked N-terminal peptides [J].

Coussot, Gaelle ;

Hawke, David H. ;

Mularz, Amanda ;

Koomen, John M. ;

Kobayashi, Ryuji .

ANALYTICAL BIOCHEMISTRY, 2007, 361 (02) :302-304

[5] AN APPROACH TO CORRELATE TANDEM MASS-SPECTRAL DATA OF PEPTIDES WITH AMINO-ACID-SEQUENCES IN A PROTEIN DATABASE [J].

ENG, JK ;

MCCORMACK, AL ;

YATES, JR .

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 1994, 5 (11) :976-989

[6] Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides [J].

Gevaert, K ;

Goethals, M ;

Martens, L ;

Van Damme, J ;

Staes, A ;

Thomas, GR ;

Vandekerckhove, J .

NATURE BIOTECHNOLOGY, 2003, 21 (05) :566-569

[7] IDENTIFYING PROTEINS FROM 2-DIMENSIONAL GELS BY MOLECULAR MASS SEARCHING OF PEPTIDE-FRAGMENTS IN PROTEIN-SEQUENCE DATABASES [J].

HENZEL, WJ ;

BILLECI, TM ;

STULTS, JT ;

WONG, SC ;

GRIMLEY, C ;

WATANABE, C .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (11) :5011-5015

[8] Structure of a new 'aspzincin' metalloendopeptidase from Grifola frondosa:: implications for the catalytic mechanism and substrate specificity based on several different crystal forms [J].

Hori, T ;

Kumasaka, T ;

Yamamoto, M ;

Nonaka, T ;

Tanaka, N ;

Hashimoto, Y ;

Ueki, T ;

Takio, K .

ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 2001, 57 :361-368

[9] Protein sequencing by matrix-assisted laser desorption ionization-postsource decay mass spectrometry analysis of the N-tris(2,4,6-trimethoxyphenyl)phosphine-acetylated tryptic digests [J].

Huang, ZH ;

Shen, TL ;

Wu, JA ;

Gage, DA ;

Watson, JT .

ANALYTICAL BIOCHEMISTRY, 1999, 268 (02) :305-317

[10] A picomole-scale method for charge derivatization of peptides for sequence analysis by mass spectrometry [J].

Huang, ZH ;

Wu, J ;

Roth, KDW ;

Yang, Y ;

Gage, DA ;

Watson, JT .

ANALYTICAL CHEMISTRY, 1997, 69 (02) :137-144

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