Liquid chromatographic determination of inositol hexanicotinate in formulated preparations

A precise and selective liquid chromatographic procedure for determining inositol hexanicotinate (IHN) in formulated preparations was developed and validated, IHN was dissolved in dimethyl sulfoxide, diluted with acetonitrile, chromatographed on a Nova-Pak C-18 column with a mobile phase of water-methanol (40 + 60, v/v), and detected at 262 nm, The correlation coefficient of the calibration curve was 0.9995 over the range 0.05-0.15 mg/mL. Overall recovery of IHN was >97%. Coefficients of variation for intra- and interday precisions were <2%.