Hepatoprotective and antiviral properties of isochlorogenic acid A from Laggera alata against hepatitis B virus infection

被引：73

作者：

Hao, Bing-Jie ^{[1
]}

Wu, Yi-Hang ^{[1
,2
]}

Wang, Jian-Guo ^{[2
]}

Hu, Shao-Qing ^{[1
]}

Keil, Dana Jasmin ^{[3
]}

Hu, Hua-Jun ^{[1
]}

Lou, Ji-Dong ^{[1
]}

Zhao, Yu ^{[4
]}

机构：

[1] China Jiliang Univ, Dept Pharm, Zhejiang Prov Key Lab Biometrol & Inspect & Quara, Coll Life Sci, Hangzhou 310018, Peoples R China

[2] Zhejiang Univ, State Key Lab Diag & Treatment Infect Dis, Affiliated Hosp 1, Coll Med, Hangzhou 310003, Zhejiang, Peoples R China

[3] TU Dortmund Univ, Dept Biochem & Chem Engn, D-44227 Dortmund, Germany

[4] Zhejiang Univ, Dept Tradit Chinese Med & Nat Drug Res, Coll Pharmaceut Sci, Hangzhou 310058, Zhejiang, Peoples R China

来源：

JOURNAL OF ETHNOPHARMACOLOGY | 2012年 / 144卷 / 01期

基金：

中国国家自然科学基金;

关键词：

Laggera alata; Isochlorogenic acid A; Anti-hepatitis B virus; Hepatoprotective; Heme oxygenase-1; D-GALACTOSAMINE; SESQUITERPENES; HEPADNAVIRUS; OXYGENASE-1; DERIVATIVES; HEME; RATS; DNA;

D O I：

10.1016/j.jep.2012.09.003

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Ethnopharmacological relevance: The aim of this study was to determine the anti-hepatitis B effect of isochlorogenic acid A isolated from Laggera alata (Asteraceae), a traditional Chinese herbal medicine. Materials and methods: The anti-hepatitis B activity of isochlorogenic acid A was evaluated by the D-galactosamine (D-GalN)-induced HL-7702 hepatocyte damage model and the HBV-transfected HepG2.2.15 cells. Results: Isochlorogenic acid A significantly improved HL-7702 hepatocyte viability and markedly inhibited the productions of HBsAg and HBeAg. The inhibitory rates of isochlorogenic acid A on the HBsAg and HBeAg expressions were 86.9% and 72.9%, respectively. In addition, isochlorogenic acid A declined markedly the content of hepatitis B virus covalently closed circular DNA (HBV cccDNA) and induced significantly the heme oxygenase-1 (HO-1) expression in HepG2.2.15 cells. Conclusions: Isochlorogenic acid A was verified to possess the potent anti-hepatitis B activity. The anti-HBV target of isochlorogenic acid A is probably associated with blocking the translation step of the HBV replication. Overexpression of HO-1 may contribute to the anti-HBV activity of isochlorogenic acid A by reducing the stability of the HBV core protein and thus blocking the refill of nuclear HBV cccDNA. Additionally, the hepatoprotective effect of isochlorogenic acid A could be achieved by its antioxidative property and induction of HO-1. (C) 2012 Elsevier Ireland Ltd. All rights reserved.

引用

页码：190 / 194

页数：5

共 25 条

[11] Heme oxygenase-1: unleashing the protective properties of heme [J].