A Small-Molecule Probe for Selective Profiling and Imaging of Monoamine OxidaseB Activities in Models of Parkinson's Disease

被引：88

作者：

Li, Lin ^{[1
,2
,3
]}

Zhang, Cheng-Wu ^{[4
]}

Ge, Jingyan ^{[1
]}

Qian, Linghui ^{[1
]}

Chai, Bing-Han ^{[4
]}

Zhu, Qing ^{[5
]}

Lee, Jun-Seok ^{[6
,7
]}

Lim, Kah-Leong ^{[4
]}

Yao, Shao Q. ^{[1
]}

机构：

[1] Natl Univ Singapore, Dept Chem, Singapore 117543, Singapore

[2] Nanjing Tech Univ, Natl Jiangsu Synergist Innovat Ctr Adv Mat, Key Lab Flexible Elect, Nanjing 211816, Jiangsu, Peoples R China

[3] Nanjing Tech Univ, Natl Jiangsu Synergist Innovat Ctr Adv Mat, Inst Adv Mat, Nanjing 211816, Jiangsu, Peoples R China

[4] Natl Inst Neurosci, Singapore 308433, Singapore

[5] Zhejiang Univ Technol, Inst Bioengn, Hangzhou 310014, Zhejiang, Peoples R China

[6] Korea Inst Sci & Technol, Mol Recognit Res Ctr, Seoul, South Korea

[7] Univ Sci & Technol, Dept Biol Chem, Seoul, South Korea

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2015年 / 54卷 / 37期

基金：

英国医学研究理事会;

关键词：

activity-based probes; imaging; monoamine oxidases; Parkinson's disease; proteomics; MAO-A; TARGET IDENTIFICATION; FLUOROGENIC PROBES; B INHIBITORS; APOPTOSIS; EXPRESSION;

D O I：

10.1002/anie.201504441

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The design of the first dual-purpose activity-based probe of monoamine oxidaseB (MAO-B) is reported. This probe is highly selective towards MAO-B, even at high MAO-A expression levels, and could sensitively report endogenous MAO-B activities by both insitu proteome profiling and live-cell bioimaging. With a built-in imaging module as part of the probe design, the probe was able to accomplish what all previously reported MAO-B imaging probes failed to do thus far: the live-cell imaging of MAO-B activities without encountering diffusion problems.

引用

页码：10821 / 10825

页数：5

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