The p53 Protein Does Not Facilitate Adenovirus Type 5 Replication in Normal Human Cells

被引：5
作者：
Chahal, Jasdave S. [1 ]
Flint, S. J. [1 ]
机构：
[1] Princeton Univ, Lewis Thomas Lab, Dept Mol Biol, Princeton, NJ 08544 USA
来源：
JOURNAL OF VIROLOGY | 2013年 / 87卷 / 10期
基金：
美国国家卫生研究院;
关键词：
E1B 55-KILODALTON PROTEIN; E4ORF6; PROTEIN; APOPTOSIS; STABILIZATION; DEGRADATION; EXPRESSION; INDUCTION; INFECTION; REQUIRES; GENES;
D O I：
10.1128/JVI.00129-13
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Although several adenovirus type 5 (Ad5) proteins prevent deleterious consequences of activation of p53, it has been reported that viral replication proceeds more efficiently when human tumor cells produce wild-type compared to mutant p53. We have now exploited RNA interference and lentiviral vectors to achieve essentially complete knockdown of p53 in normal human cells: no effects on the kinetics or efficiency of viral gene expression or production of infectious particles were observed.
引用
收藏
页码：6044 / 6046
页数：3
相关论文
共 27 条
  • [1] The Human Adenovirus Type 5 E1B 55 kDa Protein Obstructs Inhibition of Viral Replication by Type I Interferon in Normal Human Cells
    Chahal, Jasdave S.
    Qi, Ji
    Flint, S. J.
    [J]. PLOS PATHOGENS, 2012, 8 (08):
  • [2] Timely Synthesis of the Adenovirus Type 5 E1B 55-Kilodalton Protein Is Required for Efficient Genome Replication in Normal Human Cells
    Chahal, Jasdave S.
    Flint, S. J.
    [J]. JOURNAL OF VIROLOGY, 2012, 86 (06) : 3064 - 3072
  • [3] WILD-TYPE P53 MEDIATES APOPTOSIS BY E1A, WHICH IS INHIBITED BY E1B
    DEBBAS, M
    WHITE, E
    [J]. GENES & DEVELOPMENT, 1993, 7 (04) : 546 - 554
  • [4] Dix BR, 2000, CANCER RES, V60, P2666
  • [5] A third-generation lentivirus vector with a conditional packaging system
    Dull, T
    Zufferey, R
    Kelly, M
    Mandel, RJ
    Nguyen, M
    Trono, D
    Naldini, L
    [J]. JOURNAL OF VIROLOGY, 1998, 72 (11) : 8463 - 8471
  • [6] The Temporal Order of Genetic and Pathway Alterations in Tumorigenesis
    Gerstung, Moritz
    Eriksson, Nicholas
    Lin, Jimmy
    Vogelstein, Bert
    Beerenwinkel, Niko
    [J]. PLOS ONE, 2011, 6 (11):
  • [7] Adenovirus E1B 55-kilodalton protein is required for both regulation of mRNA export and efficient entry into the late phase of infection in normal human fibroblasts
    Gonzalez, R
    Huang, WY
    Finnen, R
    Bragg, C
    Flint, SJ
    [J]. JOURNAL OF VIROLOGY, 2006, 80 (02) : 964 - 974
  • [8] ENHANCED EXPRESSION OF P53 IN HUMAN-CELLS INFECTED WITH MUTANT ADENOVIRUSES
    GRAND, RJA
    GRANT, ML
    GALLIMORE, PH
    [J]. VIROLOGY, 1994, 203 (02) : 229 - 240
  • [9] p53-dependent cell death apoptosis is required for a productive adenovirus infection
    Hall, AR
    Dix, BR
    O'Carroll, SJ
    Braithwaite, AW
    [J]. NATURE MEDICINE, 1998, 4 (09) : 1068 - 1072
  • [10] Role of the RNA recognition motif of the E1B 55 kDa protein in the adenovirus type 5 infectious cycle
    Kato, Sayuri E. M.
    Huang, Wenying
    Flint, S. J.
    [J]. VIROLOGY, 2011, 417 (01) : 9 - 17
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