Expression, purification, crystallization and preliminary X- ray diffraction analysis of EtFPOX from Eupenicillium terrenum sp.

被引：7

作者：

Xing, Keke ^{[1
,2
]}

Gan, Weiqiong ^{[1
]}

Jia, Minze ^{[1
]}

Gao, Feng ^{[1
]}

Gong, Weimin ^{[1
]}

机构：

[1] Chinese Acad Sci, Inst Biophys, Lab Noncoding RNA, Beijing 100101, Peoples R China

[2] Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China

来源：

ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS | 2013年 / 69卷

基金：

美国国家科学基金会;

关键词：

FRUCTOSYL-AMINE OXIDASE; ACID OXIDASE; CLONING; SITE;

D O I：

10.1107/S1744309113012128

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The flavoenzyme fructosyl peptide oxidase (FPOX) catalyses the oxidative deglycation of fructosyl amino acids or fructosyl dipeptides to produce amino acids, glucosone and hydrogen peroxide. In this study, FPOX protein from Eupenicillium terrenum sp. (EtFPOX) was expressed in Escherichia coli and purified by Ni-affinity and gel-filtration chromatography. EtFPOX crystals were obtained using the sitting-drop vapour-diffusion method with polyethylene glycol 3350 as precipitant. X-ray diffraction data were collected to 1.90 angstrom resolution using a synchrotron-radiation source. The crystals belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 65.6, b = 80.0, c = 83.4 angstrom, and contained one molecule in the asymmetric unit. The calculated Matthews coefficient and solvent content were 2.22 angstrom (3) Da(-1) and 44.62%, respectively.

引用

页码：666 / 668

页数：3

共 50 条

[41] Crystallization and preliminary X-ray diffraction analysis of human IL-22 bound to its soluble decoy receptor IL-22BP [J].

Watanabe, Leandra ;

de Moura, Patricia Ribeiro ;

Nascimento, Alessandro Silva ;

Colau, Didier ;

Dumoutier, Laure ;

Renauld, Jean-Christophe ;

Polikarpov, Igor .

ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 2009, 65 :102-104

[42] Crystallization and preliminary X-ray analysis of the NAD+-reducing [NiFe] hydrogenase from Hydrogenophilus thermoluteolus TH-1 [J].

Taketa, Midori ;

Nakagawa, Hanae ;

Habukawa, Mao ;

Osuka, Hisao ;

Kihira, Kiyohito ;

Komori, Hirofumi ;

Shibata, Naoki ;

Ishii, Masaharu ;

Igarashi, Yasuo ;

Nishihara, Hirofumi ;

Yoon, Ki-Seok ;

Ogo, Seiji ;

Shomura, Yasuhito ;

Higuchi, Yoshiki .

ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 2015, 71 :96-99

[43] Crystallization and preliminary X-ray crystallographic analysis of MinE, the cell-division topological specificity factor from Helicobacter pylori [J].

Kang, Gil Bu ;

Song, Hye-Eun ;

Kim, Mun-Kyoung ;

Youn, Hyung-Seop ;

An, Jun Yop ;

Lee, Jung-Gyu ;

Park, Kyung Ryung ;

Eom, Soo Hyun .

ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 2010, 66 :527-529

[44] Crystallization and preliminary X-ray crystallographic analysis of L-rhamnose isomerase with a novel high thermostability from Bacillus halodurans [J].

Doan, Thi-Ngoc-Thanh ;

Prabhu, Ponnandy ;

Kim, Jin-Kwang ;

Ahn, Yeh-Jin ;

Natarajan, Sampath ;

Kang, Lin-Woo ;

Park, Geon Tae ;

Lim, Sang-Boem ;

Lee, Jung-Kul .

ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 2010, 66 :677-680

[45] Crystallization and preliminary X-ray crystallographic analysis of a helicase-like domain from a tomato mosaic virus replication protein [J].

Xiang, Hongyu ;

Niiyama, Mayumi ;

Sugiyama, Shigeru ;

Adachi, Hiroaki ;

Takano, Kazufumi ;

Murakami, Satoshi ;

Inoue, Tsuyoshi ;

Mori, Yusuke ;

Ishikawa, Masayuki ;

Matsumura, Hiroyoshi ;

Katoh, Etsuko .

ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 2011, 67 :1649-1652

[46] Overproduction, crystallization and X-ray diffraction data analysis of ectoine synthase from the cold-adapted marine bacterium Sphingopyxis alaskensis [J].

Kobus, Stefanie ;

Widderich, Nils ;

Hoeppner, Astrid ;

Bremer, Erhard ;

Smits, Sander H. J. .

ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 2015, 71 :1027-1032

[47] Crystallization and preliminary X-ray diffraction analysis of a Lys49-phospholipase A2 complexed with caffeic acid, a molecule with inhibitory properties against snake venoms [J].

Shimabuku, Patricia S. ;

Fernandes, Carlos A. H. ;

Magro, Angelo J. ;

Costa, Tassia R. ;

Soares, Andreimar M. ;

Fontes, Marcos R. M. .

ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 2011, 67 :249-252

[48] Crystallization and preliminary X-ray crystallographic analysis of BxlA, an intracellular β-D-xylosidase from Streptomyces thermoviolaceus OPC-520 [J].

Morioka, Hideaki ;

Miki, Yasuhiro ;

Saito, Kei ;

Tomoo, Koji ;

Ishida, Toshimasa ;

Hasegawa, Tomokazu ;

Yamano, Akihito ;

Takada, Chiaki ;

Miyamoto, Katsushiro ;

Tsujibo, Hiroshi .

ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 2010, 66 :791-793

[49] Crystallization and preliminary X-ray diffraction characterization of the XccFimXEAL-c-di-GMP and XccFimXEAL-c-di-GMP-XccPilZ complexes from Xanthomonas campestris [J].

Liao, Yi-Ting ;

Chin, Ko-Hsin ;

Kuo, Wei-Ting ;

Chuah, Mary Lay-Cheng ;

Liang, Zhao-Xun ;

Chou, Shan-Ho .

ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 2012, 68 :301-305

[50] Purification, crystallization and preliminary X-ray analysis of apo glyceraldehyde-3-phosphate dehydrogenase 1 (GAP1) from methicillin-resistant Staphylococcus aureus (MRSA252) [J].

Mukherjee, Somnath ;

Saha, Baisakhee ;

Dutta, Debajyoti ;

Das, Amit Kumar .

ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 2010, 66 :506-508

← 1 2 3 4 5 →