Multiplex PCR-RFLP methods for pfcrt, pfmdr1 and pfdhfr mutations in Plasmodium falciparum

被引：44
作者：
Veiga, MI
Ferreira, PE
Björkman, A
Gil, JP
机构：
[1] Karolinska Univ Hosp, Karolinska Inst, Malaria Res Unit, Div Infect Dis,Dept Med, S-17176 Stockholm, Sweden
[2] Univ Algarve, Ctr Mol & Struct Biomed, Gambelas, Portugal
来源：
MOLECULAR AND CELLULAR PROBES | 2006年 / 20卷 / 02期
关键词：
falciparum; pfmdr1; pfcrt; pfdhfr; multiplex PCR; malaria;
D O I：
10.1016/j.mcp.2005.10.003
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Plasmodium falciparum drug resistance is a major factor for the death toll of malaria. Resistance has been associated with specific single nucleotide polymorphisms (SNPs) in the parasite genes pfmdrl (N86Y) and pfcrt (K76T) associated with quionoline antimalarial resistance, and pfdhfr (N51 I, C59R. S108N) correlated with resistance of the antifolate combination sulfadoxine-pyrimethamine. These SNPs constitute the basis for the Surveillance of drug resistance through high sensitive molecular methods in malaria endemic Countries. In this work. we developed a multiplex PCR-RFLP protocols for the diagnosis of these molecular markers, leading to significant decreases in reagent costs, time, number of manipulations and hence human resources. (c) 2005 Elsevier Ltd. All rights reserved.
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收藏
页码：100 / 104
页数：5
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