A fluorescence-based optical fiber analyzer for catecholamine determination

被引：7

作者：

Silva, Lurdes ^{[1
,2
]}

Duarte, Katia ^{[3
]}

Freitas, Ana Cristina ^{[3
]}

Panteleitchouk, Teresa S. L. ^{[3
]}

Rocha-Santos, Teresa A. P. ^{[1
,2
,3
]}

Pereira, Maria E. ^{[1
,2
]}

Duarte, Armando Costa ^{[1
,2
]}

机构：

[1] Univ Aveiro, CESAM Ctr Environm & Marine Studies, P-3810193 Aveiro, Portugal

[2] Univ Aveiro, Dept Chem, P-3810193 Aveiro, Portugal

[3] ISEITIViseu, Inst Piaget, P-3515776 Lordosa, Viseu, Portugal

来源：

ANALYTICAL METHODS | 2012年 / 4卷 / 08期

关键词：

TANDEM MASS-SPECTROMETRY; PERFORMANCE LIQUID-CHROMATOGRAPHY; PLASMA-CATECHOLAMINES; METABOLITES; ELECTROPHORESIS; SEPARATION; BIOSENSOR; DOPAMINE; SAMPLES; URINE;

D O I：

10.1039/c2ay25076d

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An optical fibre (OF) analyzer for measuring catecholamines (dopamine, norepinephrine and epinephrine) in biological samples with induced fluorescence was developed. The analytical set-up included a chromatographic column for catecholamine separation and a fluorescence-based OF detection (FOF-analyzer). The detection limit of the FOF-analyser was found to be less than 0.9 pg mL(-1). The proposed methodology showed an adequate analytical performance for the determination of the catecholamines in actual samples of human urine. The analytical performance of the FOF-analyzer for catecholamine determination was investigated against the high performance liquid chromatography-electrochemical detection (HPLC-ED) method. The FOF-analyzer showed lower detection limits and larger linear ranges for determination of dopamine (DA), norepinephrine (NE) and epinephrine (E) in comparison with HPLC-ED and other methodologies such as HPLC-fluorescence. These advantages combined with the compact design, small-scale instrumentation, and effective cost of analysis make this system an interesting alternative to the existing methodologies for the determination of catecholamines in clinical samples.

引用

页码：2300 / 2306

页数：7

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