A novel fluorescent sensor for detection of highly reactive oxygen species, and for imaging such endogenous hROS in the mitochondria of living cells

被引：99

作者：

Liu, Fei ^{[1
]}

Wu, Tong ^{[1
]}

Cao, Jianfang ^{[1
]}

Zhang, Hua ^{[1
]}

Hu, Mingming ^{[1
]}

Sun, Shiguo ^{[1
]}

Song, Fengling ^{[1
]}

Fan, Jiangli ^{[1
]}

Wang, Jingyun ^{[2
]}

Peng, Xiaojun ^{[1
]}

机构：

[1] Dalian Univ Technol, State Key Lab Fine Chem, Dalian 116024, Peoples R China

[2] Dalian Univ Technol, Sch Life Sci & Biotechnol, Dalian 116024, Peoples R China

来源：

ANALYST | 2013年 / 138卷 / 03期

基金：

美国国家科学基金会;

关键词：

SELECTIVE DETECTION; HYPOCHLOROUS ACID; PROBE; DAMAGE;

D O I：

10.1039/c2an36030f

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A new dye, PTZ-Cy2, based on a hybrid cyanine-phenothiazine platform, is described. Oxidation by highly reactive oxygen species (hROS) involves attack at the thiazine sulfur atom and destruction of the pi conjugation of the cyanine moiety. Dual fluorescence emissions are enhanced dramatically at lambda(em) 470 nm and 595 nm, and PTZ-Cy2 thus serves both as an absorbance ratiometric and a fluorescent "off-on" sensor for detecting hROS. Moreover PTZ-Cy2 shows selectivity for hROS over other oxidants, and gives a highly sensitive response to such endogenous species within the mitochondria of living cells.

引用

页码：775 / 778

页数：4

共 25 条

[1] A FRET-based approach to ratiometric fluorescence detection of hydrogen peroxide
Albers, Aaron E.
Okreglak, Voytek S.
Chang, Christopher J.
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2006, 128 (30) : 9640 - 9641
[2] Neurodegenerative diseases and oxidative stress
Barnham, KJ
Masters, CL
Bush, AI
[J]. NATURE REVIEWS DRUG DISCOVERY, 2004, 3 (03) : 205 - 214
[3] FRET spectral unmixing: a ratiometric fluorescent nanoprobe for hypochlorite
Chen, Gengwen
Song, Fengling
Wang, Jingyun
Yang, Zhigang
Sun, Shiguo
Fan, Jiangli
Qiang, Xinxin
Wang, Xu
Dou, Bairui
Peng, Xiaojun
[J]. CHEMICAL COMMUNICATIONS, 2012, 48 (24) : 2949 - 2951
[4] Fluorescent and luminescent probes for detection of reactive oxygen and nitrogen species
Chen, Xiaoqiang
Tian, Xizhe
Shin, Injae
Yoon, Juyoung
[J]. CHEMICAL SOCIETY REVIEWS, 2011, 40 (09) : 4783 - 4804
[5] A highly selective and sensitive fluorescence probe for the hypochlorite anion
Chen, Xinqi
Wang, Xiaochun
Wang, Shajuan
Shi, Wen
Wang, Ke
Ma, Huimin
[J]. CHEMISTRY-A EUROPEAN JOURNAL, 2008, 14 (15) : 4719 - 4724
[6] Mitochondrial-targeted fluorescent probes for reactive oxygen species
Dickinson, Bryan C.
Srikun, Duangkhae
Chang, Christopher J.
[J]. CURRENT OPINION IN CHEMICAL BIOLOGY, 2010, 14 (01) : 50 - 56
[7] Highly reactive oxygen species: detection, formation, and possible functions
Freinbichler, Wolfhardt
Colivicchi, Maria A.
Stefanini, Chiara
Bianchi, Loria
Ballini, Chiara
Misini, Bashkim
Weinberger, Peter
Linert, Wolfgang
Vareslija, Damir
Tipton, Keith F.
Della Corte, Laura
[J]. CELLULAR AND MOLECULAR LIFE SCIENCES, 2011, 68 (12) : 2067 - 2079
[8] James AM, 2002, J BIOMED SCI, V9, P475, DOI 10.1159/000064721
[9] A BODIPY-Based Probe for the Selective Detection of Hypochlorous Acid in Living Cells
Kim, Tae-Il
Park, Seonhwa
Choi, Yongdoo
Kim, Youngmi
[J]. CHEMISTRY-AN ASIAN JOURNAL, 2011, 6 (06) : 1358 - 1361
[10] Design and synthesis of fluorescent probes for selective detection of highly reactive oxygen species in mitochondria of living cells
Koide, Yuichiro
Urano, Yasuteru
Kenmoku, Suguru
Kojima, Hirotatsu
Nagano, Tetsuo
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2007, 129 (34) : 10324 - +

← 1 2 3 →