A novel fluorescent sensor for detection of highly reactive oxygen species, and for imaging such endogenous hROS in the mitochondria of living cells

被引：99

作者：

Liu, Fei ^{[1
]}

Wu, Tong ^{[1
]}

Cao, Jianfang ^{[1
]}

Zhang, Hua ^{[1
]}

Hu, Mingming ^{[1
]}

Sun, Shiguo ^{[1
]}

Song, Fengling ^{[1
]}

Fan, Jiangli ^{[1
]}

Wang, Jingyun ^{[2
]}

Peng, Xiaojun ^{[1
]}

机构：

[1] Dalian Univ Technol, State Key Lab Fine Chem, Dalian 116024, Peoples R China

[2] Dalian Univ Technol, Sch Life Sci & Biotechnol, Dalian 116024, Peoples R China

来源：

ANALYST | 2013年 / 138卷 / 03期

基金：

美国国家科学基金会;

关键词：

SELECTIVE DETECTION; HYPOCHLOROUS ACID; PROBE; DAMAGE;

D O I：

10.1039/c2an36030f

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A new dye, PTZ-Cy2, based on a hybrid cyanine-phenothiazine platform, is described. Oxidation by highly reactive oxygen species (hROS) involves attack at the thiazine sulfur atom and destruction of the pi conjugation of the cyanine moiety. Dual fluorescence emissions are enhanced dramatically at lambda(em) 470 nm and 595 nm, and PTZ-Cy2 thus serves both as an absorbance ratiometric and a fluorescent "off-on" sensor for detecting hROS. Moreover PTZ-Cy2 shows selectivity for hROS over other oxidants, and gives a highly sensitive response to such endogenous species within the mitochondria of living cells.

引用

页码：775 / 778

页数：4

共 25 条

[1] A FRET-based approach to ratiometric fluorescence detection of hydrogen peroxide [J].

Albers, Aaron E. ;

Okreglak, Voytek S. ;

Chang, Christopher J. .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2006, 128 (30) :9640-9641

[2] Neurodegenerative diseases and oxidative stress [J].

Barnham, KJ ;

Masters, CL ;

Bush, AI .

NATURE REVIEWS DRUG DISCOVERY, 2004, 3 (03) :205-214

[3] FRET spectral unmixing: a ratiometric fluorescent nanoprobe for hypochlorite [J].

Chen, Gengwen ;

Song, Fengling ;

Wang, Jingyun ;

Yang, Zhigang ;

Sun, Shiguo ;

Fan, Jiangli ;

Qiang, Xinxin ;

Wang, Xu ;

Dou, Bairui ;

Peng, Xiaojun .

CHEMICAL COMMUNICATIONS, 2012, 48 (24) :2949-2951

[4] Fluorescent and luminescent probes for detection of reactive oxygen and nitrogen species [J].

Chen, Xiaoqiang ;

Tian, Xizhe ;

Shin, Injae ;

Yoon, Juyoung .

CHEMICAL SOCIETY REVIEWS, 2011, 40 (09) :4783-4804

[5] A highly selective and sensitive fluorescence probe for the hypochlorite anion [J].

Chen, Xinqi ;

Wang, Xiaochun ;

Wang, Shajuan ;

Shi, Wen ;

Wang, Ke ;

Ma, Huimin .

CHEMISTRY-A EUROPEAN JOURNAL, 2008, 14 (15) :4719-4724

[6] Mitochondrial-targeted fluorescent probes for reactive oxygen species [J].

Dickinson, Bryan C. ;

Srikun, Duangkhae ;

Chang, Christopher J. .

CURRENT OPINION IN CHEMICAL BIOLOGY, 2010, 14 (01) :50-56

[7] Highly reactive oxygen species: detection, formation, and possible functions [J].

Freinbichler, Wolfhardt ;

Colivicchi, Maria A. ;

Stefanini, Chiara ;

Bianchi, Loria ;

Ballini, Chiara ;

Misini, Bashkim ;

Weinberger, Peter ;

Linert, Wolfgang ;

Vareslija, Damir ;

Tipton, Keith F. ;

Della Corte, Laura .

CELLULAR AND MOLECULAR LIFE SCIENCES, 2011, 68 (12) :2067-2079

[8]

James AM, 2002, J BIOMED SCI, V9, P475, DOI 10.1159/000064721

[9] A BODIPY-Based Probe for the Selective Detection of Hypochlorous Acid in Living Cells [J].

Kim, Tae-Il ;

Park, Seonhwa ;

Choi, Yongdoo ;

Kim, Youngmi .

CHEMISTRY-AN ASIAN JOURNAL, 2011, 6 (06) :1358-1361

[10] Design and synthesis of fluorescent probes for selective detection of highly reactive oxygen species in mitochondria of living cells [J].

Koide, Yuichiro ;

Urano, Yasuteru ;

Kenmoku, Suguru ;

Kojima, Hirotatsu ;

Nagano, Tetsuo .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2007, 129 (34) :10324-+

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