Application of Nanoparticle Antioxidants to Enable Hyperstable Chloroplasts for Solar Energy Harvesting

被引:84
作者
Boghossian, Ardemis A. [1 ,2 ]
Sen, Fatih [1 ,3 ]
Gibbons, Brenna M. [5 ]
Sen, Selda [1 ,4 ]
Faltermeier, Sean M. [1 ]
Giraldo, Juan Pablo [1 ]
Zhang, Cathy T. [1 ]
Zhang, Jingqing [1 ]
Heller, Daniel A. [6 ]
Strano, Michael S. [1 ]
机构
[1] MIT, Dept Civil Engn, Cambridge, MA 02139 USA
[2] CALTECH, Dept Chem Engn, Pasadena, CA 91106 USA
[3] Dumlupinar Univ, Dept Chem, TR-43020 Kutahya, Turkey
[4] Middle E Tech Univ, Dept Chem, TR-06531 Ankara, Turkey
[5] Dartmouth Coll, Dept Chem, Hanover, NH 03755 USA
[6] Mem Sloan Kettering Canc Ctr, Mol Pharmacol & Chem Program, New York, NY 10065 USA
基金
美国国家科学基金会;
关键词
dextran-wrapped nanoceria; cerium oxide (CeO2) nanoparticle; single-walled carbon nanotube (SWCNT); fullerenol (polyhydroxylated fullerene; buckeyball; C60); 2; 7-dichlorodihydrofluorescein diacetate (H2DCF-DA); 3-bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide sodium salt (XTT); dichloroindophenol (DCPIP); reactive oxygen species (ROS); regeneration; scavenging; chloroplast; CERIUM OXIDE NANOPARTICLES; WATER-WATER CYCLE; IMMOBILIZED CHLOROPLASTS; CALCIUM DEFICIENCY; LIGHT; DAMAGE; PHOTOSYNTHESIS; CONVERSION; PROTECTION; NANOCERIA;
D O I
10.1002/aenm.201201014
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
The chloroplast contains densely stacked arrays of light-harvesting proteins that harness solar energy with theoretical maximum glucose conversion efficiencies approaching 12%. Few studies have explored isolated chloroplasts as a renewable, abundant, and low cost source for solar energy harvesting. One impediment is that photoactive proteins within the chloroplast become photodamaged due to reactive oxygen species (ROS) generation. In vivo, chloroplasts reduce photodegradation by applying a self-repair cycle that dynamically replaces photodamaged components; outside the cell, ROS-induced photodegradation contributes to limited chloroplast stability. The incorporation of chloroplasts into synthetic, light-harvesting devices will require regenerative ROS scavenging mechanisms to prolong photoactivity. Herein, we study ROS generation within isolated chloroplasts extracted from Spinacia oleracea directly interfaced with nanoparticle antioxidants, including dextran-wrapped nanoceria (dNC) previously demonstrated as a potent ROS scavenger. We quantitatively examine the effect of dNC, along with cerium ions, fullerenol, and DNA-wrapped single-walled carbon nanotubes (SWCNTs), on the ROS generation of isolated chloroplasts using the oxidative dyes, 2',7'- dichlorodihydrofluorescein diacetate (H2DCF-DA) and 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide sodium salt (XTT). Electrochemical measurements confirm that chloroplasts processed from free solution can generate power under illumination. We find dNC to be the most effective of these agents for decreasing oxidizing species and superoxide concentrations whilst preserving chloroplast photoactivity at concentrations below 5 M, offering a promising mechanism for maintaining regenerative chloroplast photoactivity for light-harvesting applications.
引用
收藏
页码:881 / 893
页数:13
相关论文
共 90 条
[1]   Use of a new tetrazolium-based assay to study the production of superoxide radicals by tobacco cell cultures challenged with avirulent zoospores of Phytophthora parasitica var nicotianae [J].
Able, AJ ;
Guest, DI ;
Sutherland, MW .
PLANT PHYSIOLOGY, 1998, 117 (02) :491-499
[2]   Combined cytotoxic and anti-invasive properties of redox-active nanoparticles in tumor-stroma interactions [J].
Alili, Lirija ;
Sack, Maren ;
Karakoti, Ajay S. ;
Teuber, Sarah ;
Puschmann, Katharina ;
Hirst, Suzanne M. ;
Reilly, Christopher M. ;
Zanger, Klaus ;
Stahl, Wilhelm ;
Das, Soumen ;
Seal, Sudipta ;
Brenneisen, Peter .
BIOMATERIALS, 2011, 32 (11) :2918-2929
[3]   Optimal photosynthetic characteristics of individual plants in vegetation stands and implications for species coexistence [J].
Anten, NPR .
ANNALS OF BOTANY, 2005, 95 (03) :495-506
[4]   COPPER ENZYMES IN ISOLATED CHLOROPLASTS - POLYPHENOLOXIDASE IN BETA-VULGARIS [J].
ARNON, DI .
PLANT PHYSIOLOGY, 1949, 24 (01) :1-15
[5]   PHOTOINHIBITION OF PHOTOSYSTEM-2 - INACTIVATION, PROTEIN DAMAGE AND TURNOVER [J].
ARO, EM ;
VIRGIN, I ;
ANDERSSON, B .
BIOCHIMICA ET BIOPHYSICA ACTA, 1993, 1143 (02) :113-134
[6]   RECOVERY FROM PHOTOINHIBITION IN PEAS (PISUM-SATIVUM L) ACCLIMATED TO VARYING GROWTH IRRADIANCES - ROLE OF D1 PROTEIN-TURNOVER [J].
ARO, EM ;
MCCAFFERY, S ;
ANDERSON, JM .
PLANT PHYSIOLOGY, 1994, 104 (03) :1033-1041
[7]  
Arora A, 2002, CURR SCI INDIA, V82, P1227
[8]   The water-water cycle in chloroplasts: Scavenging of active oxygens and dissipation of excess photons [J].
Asada, K .
ANNUAL REVIEW OF PLANT PHYSIOLOGY AND PLANT MOLECULAR BIOLOGY, 1999, 50 :601-639
[9]   Production and scavenging of reactive oxygen species in chloroplasts and their functions [J].
Asada, Kozi .
PLANT PHYSIOLOGY, 2006, 141 (02) :391-396
[10]  
Asimov I., 1968, Photosynthesis