N6-Substituted AMPs Inhibit Mammalian Deoxynucleotide N-Hydrolase DNPH1

被引：17
作者：
Amiable, Claire [1 ,2 ,3 ]
Pochet, Sylvie [1 ,2 ]
Padilla, Andre [4 ,5 ,6 ]
Labesse, Gilles [4 ,5 ,6 ]
Kaminski, Pierre Alexandre [1 ,2 ]
机构：
[1] Inst Pasteur, Unite Chim & Biocatalyse, Paris, France
[2] CNRS, UMR3523, Paris, France
[3] Univ Paris 05, Sorbonne Paris Cite, Paris, France
[4] CNRS, Ctr Biochim Structurale, UMR5048, Montpellier, France
[5] INSERM, U554, Montpellier, France
[6] Univ Montpellier 1 & 2, IFR3, Montpellier, France
来源：
PLOS ONE | 2013年 / 8卷 / 11期
关键词：
BREAST-CANCER CELLS; KINETIN-RIBOSIDE; LACTATE-DEHYDROGENASE; ANTICANCER ACTIVITY; HL-60; CELLS; RCL; APOPTOSIS; GENE; NUCLEOSIDE; ANALOGS;
D O I：
10.1371/journal.pone.0080755
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The gene dnph1 (or rcl) encodes a hydrolase that cleaves the 2'-deoxyribonucleoside 5'-monophosphate (dNMP) N-glycosidic bond to yield a free nucleobase and 2-deoxyribose 5-phosphate. Recently, the crystal structure of rat DNPH1, a potential target for anti-cancer therapies, suggested that various analogs of AMP may inhibit this enzyme. From this result, we asked whether N-6-substituted AMPs, and among them, cytotoxic cytokinin riboside 5'-monophosphates, may inhibit DNPH1. Here, we characterized the structural and thermodynamic aspects of the interactions of these various analogs with DNPH1. Our results indicate that DNPH1 is inhibited by cytotoxic cytokinins at concentrations that inhibit cell growth.
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页数：11
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