Fast and specific dermatophyte detection by automated DNA extraction and real-time PCR

被引：37

作者：

Bergman, A. ^{[1
]}

Heimer, D. ^{[2
]}

Kondori, N. ^{[3
]}

Enroth, H. ^{[1
]}

机构：

[1] Unilabs AB, Dept Clin Microbiol, S-54185 Skovde, Sweden

[2] Unilabs AB, Capio St Gorans Hosp, Dept Clin Microbiol, Stockholm, Sweden

[3] Sahlgrens Univ Hosp, Dept Clin Bacteriol, Gothenburg, Sweden

来源：

CLINICAL MICROBIOLOGY AND INFECTION | 2013年 / 19卷 / 04期

关键词：

Dermatophyte; DNA extraction; melting-point analysis; pan-dermatophyte; real-time PCR; POLYMERASE-CHAIN-REACTION; TRICHOPHYTON-RUBRUM; IDENTIFICATION; NAIL; DIAGNOSIS; ONYCHOMYCOSIS; INFECTIONS; SKIN; SPECIMENS;

D O I：

10.1111/1469-0691.12153

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

The aim of this study was to develop and validate a rapid and sensitive real-time PCR method for detection of all known species of dermatophytes, including identification of Trichophyton rubrum and Trichophyton interdigitale. Fungal DNA was extracted directly from clinical samples by using a pre-lysis step, followed by automated DNA extraction on the MagNA Pure Compact. In total, 202 clinical samples were examined by both conventional culture and by the new PCR method. In 103 (51%) of the samples fungal nucleic acid was detected by PCR, while only 79 (39%) were found to be positive by culture. Out of 103 PCR-positive clinical samples, 94 (91%) were identified as T.rubrum and eight (8%) as T.interdigitale. This real-time PCR is far more sensitive and 24weeks faster than conventional culture for detection of dermatophytes present in clinical samples.

引用

页码：E205 / E211

页数：7

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