Time-course changes in the expression of heme oxygenase-1 in human subcutaneous hemorrhage

被引：11

作者：

Nakajima, T

Hayakawa, M

Yajima, D

Motani-Saitoh, H

Sato, Y

Kiuchi, M

Ichinose, M

Iwase, H

机构：

[1] Chiba Univ, Grad Sch Med, Dept Legal Med, Chuo Ku, Chiba 2608670, Japan

[2] Chiba Univ, Grad Sch Med, Dept Plast Surg, Chuo Ku, Chiba 2608670, Japan

来源：

FORENSIC SCIENCE INTERNATIONAL | 2006年 / 158卷 / 2-3期

关键词：

heme oxygenase; subcutaneous hemorrhage; immunohistochemistry; macropahge; time-course; wound age;

D O I：

10.1016/j.forsciint.2005.05.028

中图分类号：

DF [法律]; D9 [法律]; R [医药、卫生];

学科分类号：

0301 ; 10 ;

摘要：

To determine the time-course of human subcutaneous hemorrhage, heme oxygenase (HO)-1 expression and macrophage infiltration were observed using an immunohistochemical technique and semiquantitative analysis. The number of immunoreactive cells and the number of all infiltrating cells of each microscopic field were counted, and the ratio of the former to the latter was calculated as the positive cells ratio. An increase in the HO-1-positive cells ratio was observed starting at 3 h after injury, and the maximum ratio was observed 3 days after injury. The pattern of the increase in the macrophage ratio was similar to that of the HO-1-positive cells ratio in the early period after injury. Observation of serial sections revealed that the expression of HO-1 in the cells corresponded to the localization of macrophage. The present results suggest that the determination of HO- I expression, as derived from macrophages, might be useful for the estimation of the time-course of subcutaneous hernorrhage. (c) 2005 Elsevier Ireland Ltd. All rights reserved.

引用

页码：157 / 163

页数：7

共 44 条

[1] HISTOLOGICAL AND ENZYME-HISTOCHEMICAL PARAMETERS FOR THE AGE ESTIMATION OF HUMAN SKIN WOUNDS [J].