Gene Sequencing for Routine Verification of Pyrazinamide Resistance in Mycobacterium tuberculosis: a Role for pncA but Not rpsA

被引：77

作者：

Alexander, David C. ^{[1
,2
]}

Ma, Jennifer H. ^{[1
]}

Guthrie, Jennifer L. ^{[1
]}

Blair, Joanne ^{[1
]}

Chedore, Pam ^{[1
]}

Jamieson, Frances B. ^{[1
,2
]}

机构：

[1] Publ Hlth Ontario, Publ Hlth Labs, Toronto, ON, Canada

[2] Univ Toronto, Dept Lab Med & Pathobiol, Toronto, ON, Canada

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2012年 / 50卷 / 11期

关键词：

SUSCEPTIBILITY; COMPLEX;

D O I：

10.1128/JCM.00620-12

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Pyrazinamide (PZA) is an important component of first-line therapy for the treatment of tuberculosis. Here, we evaluate targeted gene sequencing as a supplement to phenotypic PZA susceptibility testing of Mycobacterium tuberculosis. Routine sequencing of pncA, but not rpsA, is effective for verification of PZA susceptibility results.

引用

页码：3726 / 3728

页数：3

共 11 条

[1]

Becton Dickinson and Company, 2002, BACT MGIT 960 PZA KI

[2] Pyrazinamide Susceptibility Testing in Mycobacterium tuberculosis: a Systematic Review with Meta-Analyses [J].

Chang, Kwok Chiu ;

Yew, Wing Wai ;

Zhang, Ying .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2011, 55 (10) :4499-4505

[3] Potential for Erroneous Results Indicating Resistance When Using the Bactec MGIT 960 System for Testing Susceptibility of Mycobacterium tuberculosis to Pyrazinamide [J].

Chedore, Pamela ;

Bertucci, Lina ;

Wolfe, Joyce ;

Sharma, Meenu ;

Jamieson, Frances .

JOURNAL OF CLINICAL MICROBIOLOGY, 2010, 48 (01) :300-301

[4]

Clinical and Laboratory Standards Institute (CLSI), 2011, M24A2 CLSI

[5] Widespread pyrazinamide-resistant Mycobacterium tuberculosis family in a low-incidence setting [J].

Nguyen, D ;

Brassard, P ;

Westley, J ;

Thibert, L ;

Proulx, M ;

Henry, K ;

Schwartzman, K ;

Menzies, D ;

Behr, MA .

JOURNAL OF CLINICAL MICROBIOLOGY, 2003, 41 (07) :2878-2883

[6] Mutations in pncA, a gene encoding pyrazinamidase/nicotinamidase, cause resistance to the antituberculous drug pyrazinamide in tubercle bacillus [J].

Scorpio, A ;

Zhang, Y .

NATURE MEDICINE, 1996, 2 (06) :662-667

[7] Pyrazinamide Inhibits Trans-Translation in Mycobacterium tuberculosis [J].

Shi, Wanliang ;

Zhang, Xuelian ;

Jiang, Xin ;

Yuan, Haiming ;

Lee, Jong Seok ;

Barry, Clifton E., III ;

Wang, Honghai ;

Zhang, Wenhong ;

Zhang, Ying .

SCIENCE, 2011, 333 (6049) :1630-1632

[8] Validation of pncA Gene Sequencing in Combination with the Mycobacterial Growth Indicator Tube Method To Test Susceptibility of Mycobacterium tuberculosis to Pyrazinamide [J].

Simons, Sami O. ;

van Ingen, Jakko ;

van der Laan, Tridia ;

Mulder, Arnout ;

Dekhuijzen, P. N. Richard ;

Boeree, Martin J. ;

van Soolingen, Dick .

JOURNAL OF CLINICAL MICROBIOLOGY, 2012, 50 (02) :428-434

[9] Sequencing of the pncA gene in members of the Mycobacterium tuberculosis complex has important diagnostic applications:: Identification of a species-specific pncA mutation in "Mycobacterium canettii" and the reliable and rapid predictor of pyrazinamide resistance [J].

Somoskovi, Akos ;

Dormandy, Jillian ;

Parsons, Linda M. ;

Kaswa, Michel ;

Goh, Khye Seng ;

Rastogi, Nalin ;

Salfinger, Max .

JOURNAL OF CLINICAL MICROBIOLOGY, 2007, 45 (02) :595-599

[10] In-Depth Molecular Characterization of Mycobacterium tuberculosis from New Delhi - Predominance of Drug Resistant Isolates of the 'Modern' (TbD1-) Type [J].

Stavrum, Ruth ;

Myneedu, Vithal Prasad ;

Arora, Virendra K. ;

Ahmed, Niyaz ;

Grewal, Harleen M. S. .

PLOS ONE, 2009, 4 (02)

← 1 2 →