Investigations on the antimicrobial activity and wound healing potential of ZnO nanoparticles

被引:191
作者
Kaushik, M. [1 ]
Niranjan, R. [1 ]
Thangam, Ramar [2 ]
Madhan, Balaraman [2 ]
Pandiyarasan, V. [3 ]
Ramachandran, C. [4 ]
Oh, Deog-Hwan [4 ]
Venkatasubbu, G. Devanand [1 ]
机构
[1] SRM Inst Sci & Technol, Dept Nanotechnol, Kattankulathur 603203, Tamil Nadu, India
[2] CSIR Cent Leather Res Inst, Chennai, Tamil Nadu, India
[3] Indian Inst Informat Technol Design & Mfg, Dept Elect & Commun, Kancheepuram, Tamil Nadu, India
[4] Kangwon Natl Univ, Dept Food Sci & Biotechnol, Coll Biotechnol & Biosci, Hyoja, South Korea
关键词
Zinc Oxide; Annealing; NIH3T3; cells; Antimicrobial activity; F-actin; IN-VITRO; TITANIUM-DIOXIDE; A549; ACTIVITY; THIN-FILMS; ANTIBACTERIAL; HYDROXYAPATITE; SCAFFOLDS; DELIVERY; TOXICITY; PERFORMANCE;
D O I
10.1016/j.apsusc.2019.02.189
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
Zinc Oxide (ZnO) nanoparticles are used in the field of nanomedicine. ZnO is non-toxic and compatible with biological system. The major problem of wound is infections. The infections delays wound healing. Usage of Nanomaterials like ZnO nanoparticles for wound healing is promising. ZnO nanoparticles are synthesised by wet chemical method. It is annealed at 300, 500, 700, and 900 degrees C for 3 h. ZnO nanoparticle morphology and size distribution are analysed by XRD, FTIR and TEM. The influences of particle size on fibroblast cell growth (NIH3T3) and on antimicrobial activity are analysed. The growth of fibroblast cell is higher with ZnO nanoparticles of larger particle size. Further the MTT analysis, fluorescence calcein AM cell staining and F-actin analysis confirms the growth of fibroblast cells with improved biocompatibility behaviour. The antimicrobial activity is higher for ZnO nanoparticles of lower particle size. Confocal microscope analysis and Zone of inhibition analysis confirms the antimicrobial activity of ZnO nanoparticles.
引用
收藏
页码:1169 / 1177
页数:9
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