Rabbit ubiquitin-activating enzyme E1: CDNA cloning, sequence and expression

被引：7

作者：

Sun, BG ^{[1
]}

Jeyaseelan, K ^{[1
]}

Chung, MCM ^{[1
]}

Teo, TS ^{[1
]}

机构：

[1] NATL UNIV SINGAPORE,FAC MED,DEPT BIOCHEM,SINGAPORE 119260,SINGAPORE

来源：

GENE | 1997年 / 196卷 / 1-2期

关键词：

ubiquitination; protein degradation; tissue distribution;

D O I：

10.1016/S0378-1119(97)00154-6

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A cDNA clone encoding ubiquitin-activating enzyme E1 has been isolated from a rabbit heart cDNA library and sequenced. The 3.485 kb cDNA contains an open reading frame of 1058 amino acid residues which predicts a protein of approx. 118 kDa. The deduced protein sequence exhibits a very high homology to other ubiquitin-activating enzymes identified in a variety of organisms. Northern blot analysis reveals a single transcript of apporx. 3.5 kb in all the rabbit tissues examined. The entire coding region of the rabbit E1 cDNA has been expressed as a his-tagged protein. The recombinant protein has been verified by its ability to cross-react with anti-human E1 antibodies. Ubiquitin thiolester assay shows that the recombinant rabbit E1 protein is functional. (C) 1997 Elsevier Science B.V.

引用

页码：19 / 23

页数：5

共 19 条

[1] COMPLEMENTATION BY A CLONED HUMAN UBIQUITIN-ACTIVATING ENZYME E1 OF THE S-PHASE-ARRESTED MOUSE FM3A CELL MUTANT WITH THERMOLABILE E1 [J].

AYUSAWA, D ;

KANEDA, S ;

ITOH, Y ;

YASUDA, H ;

MURAKAMI, Y ;

SUGASAWA, K ;

HANAOKA, F ;

SENO, T .

CELL STRUCTURE AND FUNCTION, 1992, 17 (02) :113-122

[2]

BANERJEE A, 1993, J BIOL CHEM, V268, P5668

[3]

CHIRGWIN J, 1979, BIOCHEMISTRY-US, V1, P5294

[4] HOW ARE SUBSTRATES RECOGNIZED BY THE UBIQUITIN-MEDIATED PROTEOLYTIC SYSTEM [J].

CIECHANOVER, A ;

SCHWARTZ, AL .

TRENDS IN BIOCHEMICAL SCIENCES, 1989, 14 (12) :483-488

[5] THE UBIQUITIN-PROTEASOME PROTEOLYTIC PATHWAY [J].

CIECHANOVER, A .

CELL, 1994, 79 (01) :13-21

[6]

FINELY D, 1984, CELL, V37, P43

[7] THE UBC3 (CDC34) UBIQUITIN-CONJUGATING ENZYME IS UBIQUITINATED AND PHOSPHORYLATED IN-VIVO [J].

GOEBL, MG ;

GOETSCH, L ;

BYERS, B .

MOLECULAR AND CELLULAR BIOLOGY, 1994, 14 (05) :3022-3029

[8]

HAAS AL, 1982, J BIOL CHEM, V257, P329

[9] MOLECULAR-CLONING, SEQUENCE, AND TISSUE DISTRIBUTION OF THE HUMAN UBIQUITIN-ACTIVATING ENZYME-E1 [J].

HANDLEY, PM ;

MUECKLER, M ;

SIEGEL, NR ;

CIECHANOVER, A ;

SCHWARTZ, AL .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (01) :258-262

[10]

HANDLEYGEARHART PM, 1994, J BIOL CHEM, V269, P33171

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