Fabrication and characterization of chitosan conjugated eurycomanone nanoparticles: In vivo evaluation of the biodistribution and toxicity in fish

被引：16

作者：

Bhat, Irfan Ahmad ^{[1
]}

Nazir, Mir Ishfaq ^{[2
]}

Ahmad, Irshad ^{[3
]}

Pathakota, Gireesh-Babu ^{[1
]}

Chanu, T. I. ^{[4
]}

Goswami, Mukunda ^{[1
]}

Sundaray, J. K. ^{[5
]}

Sharma, Rupam ^{[1
]}

机构：

[1] ICAR Cent Inst Fisheries Educ, Div Fish Genet & Biotechnol, Bombay 400061, Maharashtra, India

[2] ICAR Cent Inst Fisheries Educ, Div Fish Nutr & Feed Technol, Bombay 400061, Maharashtra, India

[3] ICAR Cent Inst Fisheries Educ, Div Aquaculture, Bombay 400061, Maharashtra, India

[4] ICAR Cent Inst Fisheries Educ, Aquaculture, Balabhadrapuram 533343, Kakinada, India

[5] ICAR Cent Inst Freshwater Aquaculture, Div Fish Genet & Biotechnol, Bhubaneswar 751002, Odisha, India

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2018年 / 112卷

关键词：

Nanoparticles; SEM; FTIR; Rhodamine-6G; Testes; Catfish; GENERIC EVOLUTIONARY METHOD; PLGA NANOPARTICLES; LONGIFOLIA JACK; SEXUAL-BEHAVIOR; DRUG-DELIVERY; MALE RATS; ENCAPSULATION; RELEASE; SPERMATOGENESIS; TESTOSTERONE;

D O I：

10.1016/j.ijbiomac.2018.02.067

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Chitosan nanopartides (CNPs) have been proven considerable delivery agents due to their remarkable physicochemical properties. Present study reports the fabrication of CNPs by ionic gelation process and their characterization by different approaches. The constructed nanopartides were successfully conjugated with eurycomanone with significant entrapment efficiency. Particle size of chitosan and chitosan conjugated eurycomanone nanoparticles were 1262 nm and 130 nm respectively. Scanning electron microscopy showed that the particles were spherical in shape and well dispersed. Cross-linking between CNPs and eurycomanone (CENPs) were confirmed by Fourier-transform infrared (FTIB) spectroscopy. Fluorescent nanopartides were prepared by using Rhodamine-6G dye, characterised by SEM and confirmed for conjugation by FTIR. Biodistribution of CENPs showed the presence of fluorescent nano particles in liver, kidney, testes and brain of C magur. The toxicity of CENPs was evaluated by comparing the histological sections of catfish testes collected from treated and control group. No signs of toxicity were seen in testes after the delivery of CENPs. Molecular docking study revealed high spontaneous binding ability of chitosan with eurycomanone and aromatase enzyme. The study reports that CNPs can act as a stabilizing agent for eurycomanone formulation and could be a promising approach to increase the reproductive performance of the fishes. (C) 2018 Elsevier B.V. All rights reserved.

引用

页码：1093 / 1103

页数：11

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