Severe acute respiratory syndrome coronavirus accessory proteins 6 and 9b interact in vivo

被引：9
作者：
Calvo, Enrique [2 ]
DeDiego, Marta L. [3 ]
Garcia, Pilar [1 ]
Lopez, Juan A. [2 ]
Perez-Brena, Pilar [1 ]
Falcon, Ana [1 ]
机构：
[1] Inst Salud Carlos III, Ctr Nacl Microbiol, Unidad Virus Resp, Madrid, Spain
[2] CNIC, Unidad Prote, Madrid, Spain
[3] CSIC, CNB, Madrid, Spain
来源：
VIRUS RESEARCH | 2012年 / 169卷 / 01期
关键词：
SARS coronavirus; Protein; 6; 9b; Protein-protein interactions; LC-MS; DEPENDENT RNA-POLYMERASE; SARS-CORONAVIRUS; VIRUS; IDENTIFICATION; BATS; PHOSPHORYLATION; LOCALIZATION; REPLICATION; RESPONSES; GENOME;
D O I：
10.1016/j.virusres.2012.07.012
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
The 3'proximal one-third of the severe acute respiratory syndrome coronavirus (SARS-CoV) genome encodes the structural proteins and eight accessory proteins, including 3a, 3b, 6, 7a, 7b, 8a, 8b and 9b, varying in length from 39 to 274 aa which do not share significant homology with viral proteins of known coronaviruses. The SARS-CoV protein 6 is 63 amino acids in length and has been previously involved in virus pathogenicity and replication. To further analyze this functions, the interaction of SARS-CoV protein 6 with other viral and/or cellular factors has been analyzed during SARS-CoV infective cycle. Protein 6 immunoprecipitation from extracts of SARS-CoV infected cells and mass spectrometry analysis revealed an interaction of viral proteins 6 and 9b in biologically relevant conditions. This interaction has been reinforced by co-localization of both proteins in the cytoplasm of SARS-CoV infected cells. (C) 2012 Elsevier B.V. All rights reserved.
引用
收藏
页码：282 / 288
页数：7
相关论文
共 49 条
  • [1] Bats: Important reservoir hosts of emerging viruses
    Calisher, Charles H.
    Childs, James E.
    Field, Hume E.
    Holmes, Kathryn V.
    Schountz, Tony
    [J]. CLINICAL MICROBIOLOGY REVIEWS, 2006, 19 (03) : 531 - +
  • [2] Phosphorylation and subcellular localization of transmissible gastroenteritis virus nucleocapsid protein in infected cells
    Calvo, E
    Escors, D
    López, JA
    González, JM
    Alvarez, A
    Arza, E
    Enjuanes, L
    [J]. JOURNAL OF GENERAL VIROLOGY, 2005, 86 : 2255 - 2267
  • [3] Coronaviral hypothetical and structural proteins were found in the intestinal surface enterocytes and pneumocytes of severe acute respiratory syndrome (SARS)
    Chan, WS
    Wu, C
    Chow, SCS
    Cheung, T
    To, KF
    Leung, WK
    Chan, PKS
    Lee, KC
    Ng, HK
    Au, DMY
    Lo, AWI
    [J]. MODERN PATHOLOGY, 2005, 18 (11) : 1432 - 1439
  • [4] Specific epitopes of the structural and hypothetical proteins elicit variable humoral responses in SARS patients
    Chow, SCS
    Ho, CYS
    Tam, TTY
    Wu, C
    Cheung, T
    Chan, PKS
    Ng, MHL
    Hui, PK
    Ng, HK
    Au, DMY
    Lo, AWI
    [J]. JOURNAL OF CLINICAL PATHOLOGY, 2006, 59 (05) : 468 - 476
  • [5] Molecular interactions in the assembly of coronaviruses
    de Haan, CAM
    Rottier, PJM
    [J]. VIRUS STRUCTURE AND ASSEMBLY, 2005, 64 : 165 - 230
  • [6] Genomic Characterization of Severe Acute Respiratory Syndrome-Related Coronavirus in European Bats and Classification of Coronaviruses Based on Partial RNA-Dependent RNA Polymerase Gene Sequences
    Drexler, Jan Felix
    Gloza-Rausch, Florian
    Glende, Joerg
    Corman, Victor Max
    Muth, Doreen
    Goettsche, Matthias
    Seebens, Antje
    Niedrig, Matthias
    Pfefferle, Susanne
    Yordanov, Stoian
    Zhelyazkov, Lyubomir
    Hermanns, Uwe
    Vallo, Peter
    Lukashev, Alexander
    Mueller, Marcel Alexander
    Deng, Hongkui
    Herrler, Georg
    Drosten, Christian
    [J]. JOURNAL OF VIROLOGY, 2010, 84 (21) : 11336 - 11349
  • [7] Identification of a novel coronavirus in patients with severe acute respiratory syndrome
    Drosten, C
    Günther, S
    Preiser, W
    van der Werf, S
    Brodt, HR
    Becker, S
    Rabenau, H
    Panning, M
    Kolesnikova, L
    Fouchier, RAM
    Berger, A
    Burguière, AM
    Cinatl, J
    Eickmann, M
    Escriou, N
    Grywna, K
    Kramme, S
    Manuguerra, JC
    Müller, S
    Rickerts, V
    Stürmer, M
    Vieth, S
    Klenk, HD
    Osterhaus, ADME
    Schmitz, H
    Doerr, HW
    [J]. NEW ENGLAND JOURNAL OF MEDICINE, 2003, 348 (20) : 1967 - 1976
  • [8] Aetiology -: Koch's postulates fulfilled for SARS virus
    Fouchier, RAM
    Kuiken, T
    Schutten, M
    van Amerongen, G
    van Doornum, J
    van den Hoogen, BG
    Peiris, M
    Lim, W
    Stöhr, K
    Osterhaus, ADME
    [J]. NATURE, 2003, 423 (6937) : 240 - 240
  • [9] Severe acute respiratory syndrome coronavirus ORF6 antagonizes STAT1 function by sequestering nuclear import factors on the rough endoplasmic Reticulum/Golgi membrane
    Frieman, Matthew
    Yount, Boyd
    Heise, Mark
    Kopecky-Bromberg, Sarah A.
    Palese, Peter
    Baric, Ralph S.
    [J]. JOURNAL OF VIROLOGY, 2007, 81 (18) : 9812 - 9824
  • [10] Genetic analysis of influenza virus NS1 gene:: a temperature-sensitive mutant shows defective formation of virus particles
    Garaigorta, U
    Falcón, AM
    Ortín, J
    [J]. JOURNAL OF VIROLOGY, 2005, 79 (24) : 15246 - 15257
12345