4D holographic microscopy of zebrafish larvae microcirculation

被引：21

作者：

Donnarumma, Dario ^{[1
]}

Brodoline, Alexey ^{[1
]}

Alexandre, Daniel ^{[1
]}

Gross, Michel ^{[1
]}

机构：

[1] Univ Montpellier 2, Lab Charles Coulomb, UMR CNRS 5221, CC UM2 026, Pl Eugene Bataillon, F-34095 Montpellier, France

来源：

OPTICS EXPRESS | 2016年 / 24卷 / 23期

关键词：

CEREBRAL-BLOOD-FLOW; PARTICLE DIGITAL HOLOGRAPHY; INVERSE-PROBLEM APPROACH; GOLD NANOPARTICLES; RECONSTRUCTION; TRACKING; VELOCITY; DISEASE;

D O I：

10.1364/OE.24.026887

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

An original technique that combines digital holography, dual illumination of the sample and cleaning algorithm 3D reconstruction is proposed. It uses a standard transmission microscopy setup coupled with a digital holography detection. The technique is 4D, since it allows to determine, at each time step, the 3D locations (x, y, z) of many moving objects that scatter the dual illumination beam. The technique has been validated by imaging the microcirculation of blood in a fish larvae sample (the moving objects are thus red blood cells RBCs). Videos showing in 4D the moving RBCs superimposed with the perfused blood vessels are obtained. (C) 2016 Optical Society of America

引用

页码：26887 / 26900

页数：14

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