Determination of quinolones of veterinary use in bee products by ultra-high performance liquid chromatography-tandem mass spectrometry using a QuEChERS extraction procedure

被引:68
作者
Lombardo-Aguei, Manuel [1 ]
Garcia-Campana, Ana M. [1 ]
Gamiz-Gracia, Laura [1 ]
Cruces-Blanco, Carmen [1 ]
机构
[1] Fac Sci, Dept Analyt Chem, E-18071 Granada, Spain
关键词
Quinolones; UHPLC-MS/MS; QuEChERS; Honey; Royal jelly; Propolis; MACROLIDE ANTIBIOTIC-RESIDUES; LC-MS/MS; FLUORESCENCE DETECTION; HONEY; MILK; FLUOROQUINOLONES; CONFIRMATION; SULFONAMIDES; VALIDATION; SAMPLES;
D O I
10.1016/j.talanta.2012.02.011
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A reliable and rapid ultra high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) method has been developed for the determination of the eight quinolones of veterinary use regulated by European Union (marbofloxacin, ciprofloxacin, danofioxacin, enrofloxacin, sarafloxacin, difloxacin, flumequine and oxolinic acid). Chromatographic conditions were optimized in order to increase sample throughput and sensitivity. The antibiotics were detected by electrospray ionization in positive ion mode with multiple reaction monitoring (MRM) and MS/MS conditions were optimized in order to increase selectivity, selecting the corresponding product ions for quantification and identification. The separation was achieved in 3 min, using a Zorbax Eclipse Plus C18 column (50 mm x 2.1 mm, 1.8 mu m), with a mobile phase of 0.02% aqueous formic acid solution and acetonitrile. A dispersive solid phase extraction methodology, often referred to as the "QuEChERS" (quick, easy, cheap, effective, rugged, and safe) method, was optimized for extraction of the quinolones from honey and also it was evaluated for other bee products such as royal jelly and propolis. The method was validated for each matrix in terms of linearity, trueness, precision, limits of detection (LODs) and quantification (LOO). LODs ranged between 0.2 and 4.1 mu g kg(-1) with precision lower than 12% and satisfactory recoveries in most cases. The method was also applied for studying the occurrence of these antibiotics in several market samples. (C) 2012 Elsevier B.V. All rights reserved.
引用
收藏
页码:193 / 199
页数:7
相关论文
共 38 条
[1]   Multi-residue determination of veterinary drugs in milk by ultra-high-pressure liquid chromatography-tandem mass spectrometry [J].
Aguilera-Luiz, M. M. ;
Martinez Vidal, Jose Luis ;
Romero-Gonzalez, Roberto ;
Garrido Frenich, Antonia .
JOURNAL OF CHROMATOGRAPHY A, 2008, 1205 (1-2) :10-16
[2]  
Anastassiades M, 2003, J AOAC INT, V86, P412
[3]   Analytical strategies to determine quinolone residues in food and the environment [J].
Andreu, Vicente ;
Blasco, Cristina ;
Pico, Yolanda .
TRAC-TRENDS IN ANALYTICAL CHEMISTRY, 2007, 26 (06) :534-556
[4]  
[Anonymous], 2011, INFORM NOTIFICATIONS
[5]  
[Anonymous], 2010, Off. J. Eur. Union, VL15, P1
[6]  
Ding T, 2009, CHIN J CHROMATOGR, V27, P34
[7]  
Food safety, 2011, FOOD SAFETY FARM FOR
[8]   Comparison of several extraction techniques for multiclass analysis of veterinary drugs in eggs using ultra-high pressure liquid chromatography-tandem mass spectrometry [J].
Garrido Frenich, Antonia ;
del Mar Aguilera-Luiz, Maria ;
Martinez Vidal, Jose Luis ;
Romero-Gonzalez, Roberto .
ANALYTICA CHIMICA ACTA, 2010, 661 (02) :150-160
[9]   Tetracycline residues in royal jelly and honey by liquid chromatography tandem mass spectrometry: validation study according to Commission Decision 2002/657/EC [J].
Giannetti, L. ;
Longo, F. ;
Buiarelli, F. ;
Russo, M. V. ;
Neri, B. .
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2010, 398 (02) :1017-1023
[10]   New trends in fast and high-resolution liquid chromatography: a critical comparison of existing approaches [J].
Guillarme, Davy ;
Ruta, Josephine ;
Rudaz, Serge ;
Veuthey, Jean-Luc .
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2010, 397 (03) :1069-1082