Proteomic analysis of the effects of baicalein on colorectal cancer cells

被引：45

作者：

Huang, Wen-Shih ^{[2
,3
]}

Kuo, Yi-Hung ^{[2
,3
]}

Chin, Chih-Chien ^{[2
,3
]}

Wang, Jeng-Yi ^{[4
]}

Yu, Hong-Ren ^{[3
,5
]}

Sheen, Jiunn-Ming ^{[3
,5
]}

Tung, Shui-Yi ^{[6
]}

Shen, Chien-Heng ^{[6
]}

Chen, Te-Chuan ^{[7
]}

Sung, Mei-Lan ^{[1
]}

Liang, Hwey-Fang ^{[1
]}

Kuo, Hsing-Chun ^{[1
]}

机构：

[1] Chang Gung Univ Sci & Technol, Dept Nursing, Chiayi 61363, Taiwan

[2] Chang Gung Mem Hosp, Div Colon & Rectal Surg, Dept Surg, Chiayi, Taiwan

[3] Chang Gung Univ, Coll Med, Grad Inst Clin Med Sci, Tao Yuan, Taiwan

[4] Chang Gung Mem Hosp, Div Colon & Rectal Surg, Dept Surg, Linkou, Taiwan

[5] Kaohsiung Chang Gung Mem Hosp, Dept Pediat, Kaohsiung, Taiwan

[6] Chang Gung Univ, Dept Hepatogastroenterol, Chang Gung Mem Hosp, Coll Med, Kaohsiung, Taiwan

[7] Kaohsiung Chang Gung Mem Hosp, Div Nephrol, Kaohsiung, Taiwan

来源：

PROTEOMICS | 2012年 / 12卷 / 06期

关键词：

Baicalein; Cell biology; Colorectal cancer cells; MALDI-TOF-TOF; Proteomic differential displays; Reactive oxygen species; ACTIVATED PROTEIN-KINASE; INDUCED APOPTOSIS; HEPATOMA; ENHANCEMENT; INHIBITION; FLAVONOIDS; MIGRATION;

D O I：

10.1002/pmic.201100270

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Baicalein is the flavonoids with multiple pharmacological activities. The aim of our study was to investigate the effects of baicalein on colorectal cancer (CRC) and to recognize the targets of baicalein treatment. To better understand baicalein's target, proteomic approaches were used to purify and identify the protein substrates using 2D difference gel electrophoresis (2D SDS-PAGE) to elucidate proteins differential display. Results from this study investigate that baicalein treatment of CRC cells results in reduced cell proliferation. As a result, differential protein displays between baicalein-treated and untreated CRC were determined and validated. There were 11 differentially expressed proteins between baicalein-treated and untreated CRC. Furthermore, we demonstrate that baicalein inhibits cancer cell proliferation and reduced reactive oxygen species (ROS) by up-regulating the levels of peroxiredoxin-6 (PRDX6). Knockdown of PRDX6 in baicalein-treated CRC cells by specific small interfering RNA resulted in ROS production and proliferation, opposite of the baicalein treatment scenario as indicated by cell cycle distribution. These results illustrate that baicalein up-regulates the expression of PRDX6, which attenuates the generation of ROS and inhibits the growth of CRC cells, whereas baicalein treatment have no effect on normal epithelial cells.

引用

页码：810 / 819

页数：10

共 30 条

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