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High-density peptide arrays
被引:34
作者:
Breitling, Frank
[1
]
Nesterov, Alexander
[1
]
Stadler, Volker
[1
]
Felgenhauer, Thomas
[1
]
Bischoff, F. Ralf
[1
]
机构:
[1] German Canc Res Ctr, D-6900 Heidelberg, Germany
关键词:
SPOT-SYNTHESIS;
COMBINATORIAL SYNTHESIS;
PARALLEL;
EXPRESSION;
FABRICATION;
DISPLAY;
MICROARRAYS;
GENERATION;
ANTIGENS;
LIBRARY;
D O I:
10.1039/b819850k
中图分类号:
Q5 [生物化学];
Q7 [分子生物学];
学科分类号:
071010 ;
081704 ;
摘要:
Arrays promise to advance biology by allowing parallel screening for many different binding partners. Meanwhile, lithographic methods enable combinatorial synthesis of > 50 000 oligonucleotides per cm(2), an advance that has revolutionized the whole field of genomics. A similar development is expected for the field of proteomics, provided that affordable, very high-density peptide arrays are available. However, peptide arrays lag behind oligonucleotide arrays. This review discusses recent developments in the field with an emphasis on methods that lead to very high-density peptide arrays.
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页码:224 / 234
页数:11
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