High-density peptide arrays

被引:34
|
作者
Breitling, Frank [1 ]
Nesterov, Alexander [1 ]
Stadler, Volker [1 ]
Felgenhauer, Thomas [1 ]
Bischoff, F. Ralf [1 ]
机构
[1] German Canc Res Ctr, D-6900 Heidelberg, Germany
关键词
SPOT-SYNTHESIS; COMBINATORIAL SYNTHESIS; PARALLEL; EXPRESSION; FABRICATION; DISPLAY; MICROARRAYS; GENERATION; ANTIGENS; LIBRARY;
D O I
10.1039/b819850k
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Arrays promise to advance biology by allowing parallel screening for many different binding partners. Meanwhile, lithographic methods enable combinatorial synthesis of > 50 000 oligonucleotides per cm(2), an advance that has revolutionized the whole field of genomics. A similar development is expected for the field of proteomics, provided that affordable, very high-density peptide arrays are available. However, peptide arrays lag behind oligonucleotide arrays. This review discusses recent developments in the field with an emphasis on methods that lead to very high-density peptide arrays.
引用
收藏
页码:224 / 234
页数:11
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