Attenuated mesangial cell proliferation related to store-operated Ca2+ entry in aged rat: the role of STIM 1 and Orai 1

被引：20

作者：

Shen, Bing ^{[1
]}

Zhu, Jinhang ^{[1
]}

Zhang, Jin ^{[1
]}

Jiang, Feifei ^{[1
]}

Wang, Zhaoyi ^{[1
]}

Zhang, Yang ^{[3
]}

Li, Jie ^{[1
]}

Huang, Dake ^{[2
]}

Ke, Daoping ^{[1
]}

Ma, Rong ^{[4
]}

Du, Juan ^{[1
]}

机构：

[1] Anhui Med Univ, Dept Physiol, Hefei, Peoples R China

[2] Anhui Med Univ, Comprehens Lab, Basic Med Sch, Hefei, Peoples R China

[3] Anhui Prov Hosp, Hefei 230032, Peoples R China

[4] Univ N Texas, Hlth Sci Ctr Ft Worth, Dept Integrat Physiol, Ft Worth, TX USA

来源：

AGE | 2013年 / 35卷 / 06期

基金：

美国国家卫生研究院;

关键词：

Aging; STIM; 1; Orai; Glomerular mesangial cell; Proliferation; STROMAL INTERACTION MOLECULE-1; PROTEIN-KINASE-C; CALCIUM INFLUX; ENDOPLASMIC-RETICULUM; CHANNELS; CRAC; HOMEOSTASIS; SENSOR; TRPC4;

D O I：

10.1007/s11357-013-9511-5

中图分类号：

R592 [老年病学]; C [社会科学总论];

学科分类号：

03 ; 0303 ; 100203 ;

摘要：

Store-operated Ca2+ entry (SOCE) is a common and ubiquitous mechanism regulating Ca2+ influx into cells and participates in numerous biological processes including cell proliferation. Glomerular mesangial cells (GMCs) play a role in the regulation of the glomerular filtration rate. From a clinical point of view, many physiological functions alter with age. In the present study, we used angiotensin II, glucagon, and the sarco/endoplasmic reticulum membrane Ca2+ pump inhibitor thapsigargin to deplete the internal Ca2+ stores for the activation of SOCE. We found that SOCE was significantly attenuated in GMCs from aged (22-month-old) rats. The expression of SOCE-related components, stromal interaction molecule 1 (STIM 1) and Orai 1, in freshly isolated glomeruli notably decreased, and STIM 1 and Orai 1 puncta formation significantly reduced in primary-cultured GMCs in aged rats. Moreover, specific knockdown of STIM 1 and Orai 1 by small interfering RNA markedly suppressed SOCE and cell proliferation of GMCs isolated from young (3-month-old) rats. We conclude that the attenuation of GMCs proliferation can be attributed to the decreased SOCE partially caused by reduced expression of STIM 1 and Orai 1.

引用

页码：2193 / 2202

页数：10

共 40 条

[1] Coupling of STIM1 to store-operated Ca2+ entry through its constitutive and inducible movement in the endoplasmic reticulum [J].