Moss, SL and Twist, C. Influence of different work and rest distributions on performance and fatigue during simulated team handball match play. J Strength Cond Res 29( 10): 2697-2707, 2015-This study investigated the effect of different interchange strategies on performance and pacing strategy during a simulated team-sport protocol. Eight youth male team handball players completed 2 conditions ( LONG-work: 3 X 13: 00 minutes, rest: 8: 00 minutes; SHORT- work: 5 X 7: 48 minutes, rest: 3: 45 minutes). Participants were tested for 20- m sprint, countermovement jump, throwing performance, and heart rate ( HR) during conditions. Postcondition measures included repeated shuttle- sprint and jump ability, session rating of perceived exertion, blood lactate, and glucose. Faster sprint ( 3.87 +/- 6 0.27 seconds cf. 3.97 +/- 6 0.24 seconds, effect size [ ES] = 0.39, p = 0.03) and throwing performance ( 70.02 +/- 6 7.40 km. h21 cf. 69.04 +/- 6 5.57 km. h21, p. >0.05, ES = 20.15) occurred in SHORT compared with LONG by a " likely small" difference. Higher summated HR ( 157 +/- 21 cf. 150 +/- 15 AU) occurred in SHORT compared with LONG by a " likely small" difference ( ES = 0.37, p. >0.05). SHORT resulted in lower session rating of perceived exertion ( 224 6 45 AU cf. 282 6 35 AU, ES = 1.45, p = 0.001) and higher blood glucose ( 6.06 +/- 6 0.69 mmol. L-1 cf. 4.98 +/- 6 1.10 mmol. l21, ES = 21.17, p = 0.03) by a " most likely moderate" difference compared with LONG. Repeated shuttle sprint was better preserved after SHORT, with " moderately lower" 10 and 25 m times ( p <= 0.05). Interchange strategies using SHORT rather than LONG work and rest periods result in lower physiological load, leading to improved fatigue resistance and better preservation of high-intensity movements during matches.