Genetic effects of anorganic bovine bone (Bio-Oss®) on osteoblast-like MG63 cells

被引:31
作者
Carinci, F
Piattelli, A
Degidi, M
Palmieri, A
Perrotti, V
Scapoli, L
Martinelli, M
Laino, G
Pezzetti, F
机构
[1] Univ Chieti Pescara, Dent Clin, I-66100 Chieti, Italy
[2] Univ Ferrara, Dept Embryol & Morphol, I-44100 Ferrara, Italy
[3] Univ Bologna, Dent Clin, Bologna, Italy
[4] Univ Bologna, Inst Histol, Bologna, Italy
[5] CARISBO Fdn, Ctr Genet Mol, Bologna, Italy
[6] Univ Chieti Pescara, Sch Dent, I-66100 Chieti, Italy
[7] Univ Naples 2, Dent Clin, Naples, Italy
关键词
bone graft; in vitro; osteogenesis; tissue culture;
D O I
10.1016/j.archoralbio.2005.06.006
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
Bio-Oss (R) (Geistlich, Wolhusen, Switzerland) is composed by anorganic bovine bone and is widely used in several bone regeneration procedures in oral surgery. How this biornaterial alters osteobtast gene expression to promote bone formation is poorly understood. We therefore attempted to address this question by using microarray techniques to identify genes that are differentially regulated in osteoblasts exposed to Bio-Oss (R). By using DNA microarrays containing 20,000 genes, we identified in osteoblast-tike cells line (MG-63) cultured with Bio-Oss (R) several genes which expression was significantly up- and down-regulated. The differentially expressed genes cover a broad range of functional activities: (a) signaling transduction, (b) transcription, (c) cell cycle regulation, (d) vesicular transport, (e) apoptosis, and (f) immunity. These results could explain the reported bioaffinity of Bio-Oss (R) to host animals, its biological affinity to osteogenic cells and its capability to stimulate osteoblastic differentiation. The data reported are, to our knowledge, the first genetic portrait of Bio-Oss (R) effects. They can be relevant to our improved understanding of the molecular mechanism underlying bone regenerative procedures and as a model for comparing other materials with similar clinical effects. (c) 2005 Elsevier Ltd. All rights reserved.
引用
收藏
页码:154 / 163
页数:10
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