Marker evaluation for differentiation of blood and menstrual fluid by methylation-sensitive SNaPshot analysis

被引：10

作者：

Holtkoetter, Hannah ^{[1
]}

Schwender, Kristina ^{[1
]}

Wiegand, Peter ^{[2
]}

Pfeiffer, Heidi ^{[1
]}

Vennemann, Marielle ^{[1
]}

机构：

[1] Univ Munster, Inst Legal Med, Rontgenstr 23, D-48149 Munster, Germany

[2] Univ Ulm, Inst Legal Med, Albert Einstein Allee 11, D-89081 Ulm, Germany

来源：

INTERNATIONAL JOURNAL OF LEGAL MEDICINE | 2018年 / 132卷 / 02期

关键词：

Body fluids; Blood; Menstrual fluid; Vaginal secretions; Epigenetic markers; DNA methylation; BODY-FLUID; DNA; IDENTIFICATION; CONSTRUCTION; VALIDATION; EXERCISE; ASSAY;

D O I：

10.1007/s00414-018-1770-3

中图分类号：

DF [法律]; D9 [法律]; R [医药、卫生];

学科分类号：

0301 ; 10 ;

摘要：

The differentiation of blood and menstrual fluid is especially important in cases of alleged sexual assault. While the identification of blood is relatively straightforward, the identification of menstrual fluid in trace evidence has been shown to be more challenging. This may be due to the complex nature of menstrual fluid that leads to intra- and inter-individual differences in composition. Nevertheless, recent advances in DNA methylation profiling have revealed promising markers for the differentiation of the two body fluids and furthermore, markers to distinguish menstrual fluid from vaginal fluid. A literature study was performed and in total, 11 markers were evaluated in this study of which seven could be validated for menstrual fluid and blood identification purposes. Marker "BLU2" (chr16:29757334) was identified as most suitable for differentiation of blood and menstrual fluid.

引用

页码：387 / 395

页数：9

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