Vitamin C Enhances the In vitro Development of Porcine Pre-implantation Embryos by Reducing Oxidative Stress

被引:69
作者
Hu, J. [1 ,2 ]
Cheng, D. [1 ]
Gao, X. [1 ]
Bao, J. [1 ]
Ma, X. [1 ]
Wang, H. [1 ]
机构
[1] NW A&F Univ, Coll Vet Med, Yangling 712100, Shaanxi, Peoples R China
[2] Yulin Univ, Coll Life Sci, Yulin, Shaanxi, Peoples R China
基金
中国国家自然科学基金;
关键词
CELL NUCLEAR TRANSFER; OXYGEN-TENSION; APOPTOSIS; PARTHENOTES; GLUTATHIONE; ACTIVATION; EXPRESSION; PROTECTION; SELENIUM; INVITRO;
D O I
10.1111/j.1439-0531.2011.01982.x
中图分类号
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号
0905 ;
摘要
Contents Vitamin C (Vc) is a natural compound supplemented to culture media to guarantee the appropriate reactive oxygen species (ROS) level, as well as protect cells from oxidative damage and apoptosis. The current study was conducted to determine the effects of Vc (0, 2.5, 5, 10, 20 and 40 mu g/ml) on the ROS production, developmental ability and quality of in vitro produced porcine parthenotes. The results show that: (i) the ROS levels in the embryos significantly decrease in the Vc-treated groups compared with the control (p < 0.05), (ii) the rates of blastocyst formation and total cell numbers in each blastocyst are significantly higher in the Vc-treated groups than in the control (p < 0.05); the optimum concentration of Vc is 20 mu g/ml, (iii) the relative expression of Bcl-xL significantly increases and that of Bax is downregulated after Vc treatment. Terminal deoxynucleotidyl transferase-mediated dUTP nick-end labelling analysis indicates that the ratio of apoptotic cells in the blastocyst is also significantly lower in Vc-treated groups (p < 0.05) and (iv) Vc treatment can also increase the expression of the Nanog gene in porcine embryos, with a fivefold increase in 20 mu g/ml Vc treatment compared with the control (p < 0.05). Therefore, Vc improves the development of porcine embryos by reducing the ROS levels. Vc addition in PZM-3 medium can decrease the number of apoptotic cells and increase the cell numbers in blastocysts to produce high-quality porcine embryos in vitro.
引用
收藏
页码:873 / 879
页数:7
相关论文
共 21 条
[1]   Nanog safeguards pluripotency and mediates germline development [J].
Chambers, Ian ;
Silva, Jose ;
Colby, Douglas ;
Nichols, Jennifer ;
Nijmeijer, Bianca ;
Robertson, Morag ;
Vrana, Jan ;
Jones, Ken ;
Grotewold, Lars ;
Smith, Austin .
NATURE, 2007, 450 (7173) :1230-U8
[2]   Matters of life and death: programmed cell death at Cold Spring Harbor [J].
Cory, S ;
Adams, JM .
BIOCHIMICA ET BIOPHYSICA ACTA-REVIEWS ON CANCER, 1998, 1377 (02) :R25-R44
[3]   Insulin-like growth factor-I alters apoptosis related genes and reduces apoptosis in porcine parthenotes developing in vitro [J].
Cui, XS ;
Jeong, YJ ;
Jun, JH ;
Kim, NH .
THERIOGENOLOGY, 2005, 63 (04) :1070-1080
[4]   Vitamin C Enhances the Generation of Mouse and Human Induced Pluripotent Stem Cells [J].
Esteban, Miguel Angel ;
Wang, Tao ;
Qin, Baoming ;
Yang, Jiayin ;
Qin, Dajiang ;
Cai, Jinglei ;
Li, Wen ;
Weng, Zhihui ;
Chen, Jiekai ;
Ni, Su ;
Chen, Keshi ;
Li, Yuan ;
Liu, Xiaopeng ;
Xu, Jianyong ;
Zhang, Shiqiang ;
Li, Feng ;
He, Wenzhi ;
Labuda, Krystyna ;
Song, Yancheng ;
Peterbauer, Anja ;
Wolbank, Susanne ;
Redl, Heinz ;
Zhong, Mei ;
Cai, Daozhang ;
Zeng, Lingwen ;
Pei, Duanqing .
CELL STEM CELL, 2010, 6 (01) :71-79
[5]   Bax-induced caspase activation and apoptosis via cytochrome c release from mitochondria is inhibitable by Bcl-xL [J].
Finucane, DM ;
Bossy-Wetzel, E ;
Waterhouse, NJ ;
Cotter, TG ;
Green, DR .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (04) :2225-2233
[6]   Oxidative stress and protection against reactive oxygen species in the pre-implantation embryo and its surroundings [J].
Guérin, P ;
El Mouatassim, S ;
Ménézo, Y .
HUMAN REPRODUCTION UPDATE, 2001, 7 (02) :175-189
[7]   Role of nonessential amino acids on porcine embryos produced by parthenogenesis or somatic cell nuclear transfer [J].
Gupta, Mukesh Kumar ;
Uhm, Sang Jun ;
Lee, Sung-Ho ;
Lee, Hoon Taek .
MOLECULAR REPRODUCTION AND DEVELOPMENT, 2008, 75 (04) :588-597
[8]   Embryo quality and production efficiency of porcine parthenotes is improved by phytohemagglutinin [J].
Gupta, Mukesh Kumar ;
Uhm, Sang Jun ;
Han, Dong Wook ;
Lee, Hoon Taek .
MOLECULAR REPRODUCTION AND DEVELOPMENT, 2007, 74 (04) :435-444
[9]  
Hu JH, 2011, ZYGOTE, V19, P191, DOI 10.1017/S0967199410000663
[10]   Vitamin C enhances in vitro and in vivo development of porcine somatic cell nuclear transfer embryos [J].
Huang, Yongye ;
Tang, Xiaochun ;
Xie, Wanhua ;
Zhou, Yan ;
Li, Dong ;
Zhou, Yang ;
Zhu, Jianguo ;
Yuan, Ting ;
Lai, Liangxue ;
Pang, Daxin ;
Ouyang, Hongsheng .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2011, 411 (02) :397-401