Biocidal Inactivation of Lactococcus lactis Bacteriophages: Efficacy and Targets of Commonly Used Sanitizers

被引：26

作者：

Hayes, Stephen ^{[1
]}

Murphy, James ^{[1
]}

Mahony, Jennifer ^{[1
,2
]}

Lugli, Gabriele A. ^{[3
]}

Ventura, Marco ^{[3
]}

Noben, Jean-Paul ^{[4
]}

Franz, Charles M. A. P. ^{[5
]}

Neve, Horst ^{[6
]}

Nauta, Arjen

Van Sinderen, Douwe ^{[1
,2
]}

机构：

[1] Univ Coll Cork, Sch Microbiol, Cork, Ireland

[2] Univ Coll Cork, APC Microbiome Inst, Cork, Ireland

[3] Univ Parma, Dept Life Sci, Lab Probiogen, Parma, Italy

[4] Hasselt Univ, Biomed Res Inst, Diepenbeek, Belgium

[5] Max Rubner Inst, Dept Microbiol & Biotechnol, Kiel, Germany

[6] Friesland Campina, Amersfoort, Netherlands

来源：

FRONTIERS IN MICROBIOLOGY | 2017年 / 8卷

基金：

爱尔兰科学基金会;

关键词：

phage; fermentaion; starter culture; dairy; resistance; chemical; LACTOBACILLUS-PARACASEI BACTERIOPHAGES; HYDROGEN-PEROXIDE; ACID BACTERIA; MOLECULAR CHARACTERIZATION; DAIRY BACTERIOPHAGES; FUNCTIONAL-ANALYSIS; SEQUENCE-ANALYSIS; PHAGE RESISTANCE; DISINFECTANTS; PROTEINS;

D O I：

10.3389/fmicb.2017.00107

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Lactococcus lactis strains, being intensely used in the dairy industry, are particularly vulnerable to members of the so-called 936 group of phages. Sanitization and disinfection using purpose-made biocidal solutions is a critical step in controlling phage contamination in such dairy processing plants. The susceptibility of 36 936 group phages to biocidal treatments was examined using 14 biocides and commercially available sanitizers. The targets of a number of these biocides were investigated by means of electron microscopic and proteomic analyses. The results from this study highlight significant variations in phage resistance to biocides among 936 phages. Furthermore, rather than possessing resistance to specific biocides or biocide types, biocide-resistant phages tend to possess a broad tolerance to multiple classes of antimicrobial compounds.

引用

页数：14

共 53 条

[41]

Neve H, 1996, KIELER MILCHW FORSCH, V48, P359

[42] Inhibitory and bactericidal effects of hydrogen peroxide production by Streptococcus pneumoniae on other inhabitants of the upper respiratory tract [J].