Identification and characterization of nuclear microsatellite loci in Abies alba Mill.

被引：40

作者：

Cremer, E.

Liepelt, S.

Sebastiani, F.

Buonamici, A.

Michalczyk, I. M.

Ziegenhagen, B.

Vendramin, G. G.

机构：

[1] Univ Marburg, Fac Biol, Conservat Biol, D-35032 Marburg, Germany

[2] Univ Florence, Dipartimento Biotecnol Agr Genexpress, I-50019 Sesto Fiorentino, Firenze, Italy

[3] CNR, Ist Genet Vegetale, I-50019 Sesto Fiorentino, Firenze, Italy

来源：

MOLECULAR ECOLOGY NOTES | 2006年 / 6卷 / 02期

关键词：

Abies; cross-species amplification; SSR;

D O I：

10.1111/j.1471-8286.2005.01238.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Eleven polymorphic nuclear microsatellite markers for Abies alba Mill. were developed from an enriched genomic library. An average of 5.2 alleles per locus and a mean expected heterozygosity of 0.532 were found in a sample of 24 Abies alba individuals from different populations within Europe. These loci can be used in studies of genetic diversity for parentage analysis and for estimation of gene flow in silver fir populations. Moreover, successful amplifications were obtained for eight other Mediterranean Abies species, suggesting that these loci may be useful for similar applications in other fir species.

引用

页码：374 / 376

页数：3

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