Preparation and evaluation of dodecyl bonded zirconia stationary phase for high performance liquid chromatography

The C-12-alkyl phase was synthesized for zirconia substrate. The chromatographic properties of the stationary phase have been investigated by reversed-phase high-performance liquid chromatography with polycyclic aromatic hydrocarbon (PAH), alkylbenzenes, anilines, pyridines and phenols as probes. The influence of organic modifier composition was studied. The new stationary phase, similar to a silica-based reversed-phase stationary phase, has hydrophobic properties. The chromatographic behavior of the basic solutes depends mainly on hydrophobic interactions between the solutes and the hydrophobic moiety of the stationary phase. Lewis acidic sites on the surface of the stationary phase play an important role in the retention of phenols.