Temporal and spectral characterization of the photosynthetic reaction center from Heliobacterium modesticaldum

被引:21
作者
Chauvet, Adrien [1 ]
Sarrou, Josephine [2 ]
Lin, Su [2 ,3 ]
Romberger, Steven P. [4 ,5 ]
Golbeck, John H. [4 ,5 ]
Savikhin, Sergei [1 ]
Redding, Kevin E. [2 ]
机构
[1] Purdue Univ, Dept Phys, W Lafayette, IN 47907 USA
[2] Arizona State Univ, Dept Chem & Biochem, Tempe, AZ 85287 USA
[3] Arizona State Univ, Biodesign Inst, Tempe, AZ 85287 USA
[4] Penn State Univ, Dept Biochem & Mol Biol, University Pk, PA 16802 USA
[5] Penn State Univ, Dept Chem, University Pk, PA 16802 USA
基金
美国国家科学基金会;
关键词
Heliobacterium modesticaldum; Reaction center; Electrochromic shift; Accessory pigment; Bacteriochlorophyll g; I REACTION-CENTER; CYANOBACTERIAL PHOTOSYSTEM-I; SECONDARY-ELECTRON ACCEPTOR; TIME-RESOLVED SPECTROSCOPY; PRIMARY CHARGE SEPARATION; HELIOBACILLUS-MOBILIS; ENERGY-TRANSFER; TRANSIENT ABSORPTION; MEMBRANES; CHLORUM;
D O I
10.1007/s11120-013-9871-9
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
A time-resolved spectroscopic study of the isolated photosynthetic reaction center (RC) from Heliobacterium modesticaldum reveals that thermal equilibration of light excitation among the antenna pigments followed by trapping of excitation and the formation of the charge-separated state P-800 (+)A(0) (-) occurs within similar to 25 ps. This time scale is similar to that reported for plant and cyanobacterial photosystem I (PS I) complexes. Subsequent electron transfer from the primary electron acceptor A(0) occurs with a lifetime of similar to 600 ps, suggesting that the RC of H. modesticaldum is functionally similar to that of Heliobacillus mobilis and Heliobacterium chlorum. The (A(0) (-) - A(0)) and (P-800 (+) - P-800) absorption difference spectra imply that an 8(1)-OH-Chl a (F) molecule serves as the primary electron acceptor and occupies the position analogous to ec3 (A(0)) in PS I, while a monomeric BChl g pigment occupies the position analogous to ec2 (accessory Chl). The presence of an intense photobleaching band at 790 nm in the (A(0) (-) - A(0)) spectrum suggests that the excitonic coupling between the monomeric accessory BChl g and the 8(1)-OH-Chl a (F) in the heliobacterial RC is significantly stronger than the excitonic coupling between the equivalent pigments in PS I.
引用
收藏
页码:1 / 9
页数:9
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