Cross-talk reduction in confocal images of dual fluorescence labelled cell spheroids

被引：9

作者：

Ahammer, H ^{[1
]}

DeVaney, TTJ ^{[1
]}

Hartbauer, M ^{[1
]}

Tritthart, HA ^{[1
]}

机构：

[1] Graz Univ Technol, Inst Med Phys & Biophys, A-8010 Graz, Austria

来源：

MICRON | 1999年 / 30卷 / 04期

关键词：

confocal microscopy; subtraction; image processing; image enhancement; cancer; confrontation;

D O I：

10.1016/S0968-4328(99)00029-3

中图分类号：

TH742 [显微镜];

学科分类号：

摘要：

Dual fluorescence labelling is an advanced method to separate two individual specimens in a biological system using confocal microscopy. An inherent problem of this method is fluorescence channel cross-talk, which causes problems for the exact spatial determination and separation of the specimens. Using a parallel fluorescence detection and an image processing technique, based on an image subtraction method, we have developed a very straight forward method for correcting the dual channel fluorescence images. We successfully applied this method to a 3-dimensional cancer spheroid invasion assay and controlled the cross-talk compensation efficiency by a quality parameter. (C) 1999 Elsevier Science Ltd. All rights reserved.

引用

页码：309 / 317

页数：9

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