Detection of glycated hemoglobin (HbA1c) based on impedance measurement with parallel electrodes integrated into a microfluidic device

被引：37
作者：
Chuang, Ya-Chun
Lan, Kung-Chieh
Hsieh, Ku-Meng
Jang, Ling-Sheng
Chen, Ming-Kun
机构：
[1] Natl Cheng Kung Univ, Dept Elect Engn, Tainan 70101, Taiwan
[2] Natl Cheng Kung Univ, Ctr Micro Nano Sci & Technol, Tainan 70101, Taiwan
来源：
SENSORS AND ACTUATORS B-CHEMICAL | 2012年 / 171卷
关键词：
Glycated hemoglobin; Microfluidics; Self-assembled monolayer (SAM); HbA1c biosensor; Boronic affinity; SELF-ASSEMBLED MONOLAYER; THIOCTIC ACID; GOLD SURFACE; DOUBLE-LAYER; BINDING; PROTEIN; RECOGNITION; BIOSENSOR;
D O I：
10.1016/j.snb.2012.06.084
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
This paper proposes a sensor for glycated hemoglobin (HbA1c) detection based on impedance measurement. The sensor contains a pair of parallel facing electrodes integrated into a microfluidic device. A pair of parallel facing electrodes is fabricated to characterize the impedance change and determine the concentration of HbA(1c). A self-assembled monolayer (SAM) of thiophene-3-boronic acid (T3BA) is used for modifying the gold electrodes. Non-faradaic electrochemical detection of surface-bound HbA(1c) with no redox indicator added is conducted by utilizing the capacitive property as a sensing signal. The performance of the HbA(1c) sensor was investigated by impedance analyzer and the impedance changes approximately logarithmically with the concentration of HbA1c in the range of 10-100 ng/mu L.The proposed detection method has potential for use in point-of-care diagnostics and can be used for the detection of various (c) 2012 Elsevier B.V. All rights reserved.
引用
收藏
页码：1222 / 1230
页数：9
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