Analysis of cannabinoids in oral fluid by liquid chromatography-tandem mass spectrometry

被引：29
作者：
Fabritius, Marie [1 ]
Staub, Christian [1 ]
Mangin, Patrice [2 ]
Giroud, Christian [1 ]
机构：
[1] Univ Ctr Legal Med, Forens Toxicol & Chem Unit, CH-1011 Lausanne, Switzerland
[2] Univ Ctr Legal Med, Unit Forens Med, CH-1011 Lausanne, Switzerland
来源：
FORENSIC TOXICOLOGY | 2013年 / 31卷 / 01期
基金：
瑞士国家科学基金会;
关键词：
Tetrahydrocannabinol; Cannabinoids; Oral fluid; Saliva; Tandem mass spectrometry; Beta-glucuronidase; QUANTITATIVE ANALYTICAL PROCEDURES; HUMAN SERUM-ALBUMIN; MAJOR METABOLITES; GC-MS; 11-NOR-DELTA(9)-TETRAHYDROCANNABINOL-9-CARBOXYLIC ACID; TETRAHYDROCANNABINOL GLUCURONIDE; SFSTP PROPOSAL; DELTA(9)-TETRAHYDROCANNABINOL; MECONIUM; PLASMA;
D O I：
10.1007/s11419-012-0168-z
中图分类号：
R99 [毒物学（毒理学）];
学科分类号：
100405 ;
摘要：
A sensitive method was developed for quantifying a wide range of cannabinoids in oral fluid (OF) by liquid chromatography-tandem mass spectrometry (LC-MS/MS). These cannabinoids include a dagger(9)-tetrahydrocannabinol (THC), 11-hydroxy-a dagger(9)-tetrahydrocannabinol (11-OH-THC), 11-nor-9-carboxy-a dagger(9)-tetrahydrocannabinol (THCCOOH), cannabinol (CBN), cannabidiol (CBD), a dagger(9)-tetrahydrocannabinolic acid A (THC-A), 11-nor-9-carboxy-a dagger(9)-tetrahydrocannabinol glucuronide (THCCOOH-gluc), and a dagger(9)-tetrahydrocannabinol glucuronide (THC-gluc). Samples were collected using a Quantisal (TM) device. The advantages of performing a liquid-liquid extraction (LLE) of KCl-saturated OF using heptane/ethyl acetate versus a solid-phase extraction (SPE) using HLB copolymer columns were determined. Chromatographic separation was achieved in 11.5 min on a Kinetex (TM) column packed with 2.6-mu m core-shell particles. Both positive (THC, 11-OH-THC, CBN, and CBD) and negative (THCCOOH, THC-gluc, THCCOOH-gluc, and THC-A) electrospray ionization modes were employed with multiple reaction monitoring using a high-end AB Sciex API 5000 (TM) triple quadrupole LC-MS/MS system. Unlike SPE, LLE failed to extract THC-gluc and THCCOOH-gluc. However, the LLE method was more sensitive for the detection of THCCOOH than the SPE method, wherein the limit of detection (LOD) and limit of quantification (LOQ) decreased from 100 to 50 pg/ml and from 500 to 80 pg/ml, respectively. The two extraction methods were successfully applied to OF samples collected from volunteers before and after they smoked a homemade cannabis joint. High levels of THC were measured soon after smoking, in addition to significant amounts of THC-A. Other cannabinoids were found in low concentrations. Glucuronide conjugate levels were lower than the method's LOD for most samples. Incubation studies suggest that glucuronides could be enzymatically degraded by glucuronidase prior to OF collection.
引用
收藏
页码：151 / 163
页数：13
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