Two-photon laser scanning microscopy integrated with light stimulus system

被引：0
作者：
Xu, Xin [1 ,2 ]
Jin, Xingjie [1 ]
Gao, Feng [1 ]
Shi, Guohua [1 ]
机构：
[1] Chinese Acad Sci, Jiangsu Key Lab Med Opt, Suzhou Inst Biomed Engn & Technol, Suzhou 215163, Peoples R China
[2] Univ Elect Sci & Technol China, Sch Optoelect Sci & Engn, Chengdu 610052, Sichuan, Peoples R China
来源：
FIFTH SYMPOSIUM ON NOVEL OPTOELECTRONIC DETECTION TECHNOLOGY AND APPLICATION | 2019年 / 11023卷
关键词：
Two-photon microscopy; light stimulus; light intensity modulation; laser scanning microscopy;
D O I：
10.1117/12.2521012
中图分类号：
O43 [光学];
学科分类号：
070207 ; 0803 ;
摘要：
We demonstrate a home-made two-photon laser scanning microscopy (TPLSM) with a light stimulus system. In this system, the femtosecond pulses are produced by a picosecond fiber laser with pulse width compression. A laser diode serves as stimulus, which modulated by an AOM and coupled into the same light path of a femtosecond laser. The control signal of AOM and trigger signal of two scanning mirrors are synchronized by a FPGA board. With modulating the intensity of CW laser at precise point in one scanning frame, any pattern of light stimulus can be delivered to the sample in real time.
引用
收藏
页数：5
相关论文
共 50 条
[31]   Three-dimensional differentiation of photo-autotrophic biofilm constituents by multi-channel laser scanning microscopy (single-photon and two-photon excitation) [J].
Neu, TR ;
Woelfl, S ;
Lawrence, JR .
JOURNAL OF MICROBIOLOGICAL METHODS, 2004, 56 (02) :161-172
[32]   Parallel and Flexible Imaging using Two-photon RESOLFT Microscopy with Spatial Light Modulator Control [J].
Xue, Yi ;
Rowlands, Christopher J. ;
So, Peter T. C. .
MULTIPHOTON MICROSCOPY IN THE BIOMEDICAL SCIENCES XV, 2015, 9329
[33]   Two-photon scanned light sheet fluorescence microscopy with axicon imaging for fast volumetric imaging [J].
Lin, Po-Yen ;
Hwang, Sheng-Ping L. ;
Lee, Chi-Hon ;
Chen, Bi-Chang .
JOURNAL OF BIOMEDICAL OPTICS, 2021, 26 (11)
[34]   High-throughput spatial light modulation two-photon microscopy for fast functional imaging [J].
Pozzi, Paolo ;
Gandolfi, Daniela ;
Tognolina, Marialuisa ;
Chirico, Giuseppe ;
Mapelli, Jonathan ;
D'Angelo, Egidio .
NEUROPHOTONICS, 2015, 2 (01)
[35]   Quantitative 3D comparison of biofilm imaged by X-ray micro-tomography and two-photon laser scanning microscopy [J].
Larue, A. E. ;
Swider, P. ;
Duru, P. ;
Daviaud, D. ;
Ouintard, M. ;
Davit, Y. .
JOURNAL OF MICROSCOPY, 2018, 271 (03) :302-314
[36]   Bioluminescence imaging and two-photon microscopy guided laser ablation of GBM decreases tumor burden [J].
Fan, Yingwei ;
Sun, Yu ;
Chang, Wei ;
Zhang, Xinran ;
Tang, Jie ;
Zhang, Liwei ;
Liao, Hongen .
THERANOSTICS, 2018, 8 (15) :4072-4085
[37]   Analyses of bone modeling and remodeling using in vitro reconstitution system with two-photon microscopy [J].
Hikita, Atsuhiko ;
Iimura, Tadahiro ;
Oshima, Yusuke ;
Saitou, Takashi ;
Yamamoto, Shin ;
Imamura, Takeshi .
BONE, 2015, 76 :5-17
[38]   Evaluation of compact pulsed lasers for two-photon microscopy using a simple method for measuring two-photon excitation efficiency [J].
Saidi, Samir ;
Shtrahman, Matthew .
NEUROPHOTONICS, 2023, 10 (04)
[39]   A compact two-photon laser-scanning microscope made from minimally modified commercial components [J].
Iyer, V ;
Hoogland, TM ;
Losavio, BE ;
McQuiston, AR ;
Saggau, P .
MULTIPHOTON MICROSCOPY IN THE BIOMEDICAL SCIENCES II, 2002, 4620 :274-280
[40]   Use of laser scanning confocal and two-photon FRET microscopy to image and quantify the co-localization of fluorophore labeled ligands in MDCK epithelial cells [J].
Wallrabe, H ;
Elangovan, M ;
Periasamy, A ;
Barroso, MG .
MULTIPHOTON MICROSCOPY IN THE BIOMEDICAL SCIENCES, 2001, 4262 :373-382
12345