Two-photon laser scanning microscopy integrated with light stimulus system

被引：0
|
作者：
Xu, Xin [1 ,2 ]
Jin, Xingjie [1 ]
Gao, Feng [1 ]
Shi, Guohua [1 ]
机构：
[1] Chinese Acad Sci, Jiangsu Key Lab Med Opt, Suzhou Inst Biomed Engn & Technol, Suzhou 215163, Peoples R China
[2] Univ Elect Sci & Technol China, Sch Optoelect Sci & Engn, Chengdu 610052, Sichuan, Peoples R China
来源：
FIFTH SYMPOSIUM ON NOVEL OPTOELECTRONIC DETECTION TECHNOLOGY AND APPLICATION | 2019年 / 11023卷
关键词：
Two-photon microscopy; light stimulus; light intensity modulation; laser scanning microscopy;
D O I：
10.1117/12.2521012
中图分类号：
O43 [光学];
学科分类号：
070207 ; 0803 ;
摘要：
We demonstrate a home-made two-photon laser scanning microscopy (TPLSM) with a light stimulus system. In this system, the femtosecond pulses are produced by a picosecond fiber laser with pulse width compression. A laser diode serves as stimulus, which modulated by an AOM and coupled into the same light path of a femtosecond laser. The control signal of AOM and trigger signal of two scanning mirrors are synchronized by a FPGA board. With modulating the intensity of CW laser at precise point in one scanning frame, any pattern of light stimulus can be delivered to the sample in real time.
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页数：5
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