Pathogenesis of glucocorticoid-induced avascular necrosis: A microarray analysis of gene expression in vitro

被引:26
作者
Bian, Yanyan [1 ,2 ]
Qian, Wenwei [1 ,2 ]
Li, Hongling [3 ]
Zhao, Robert Chunhua [3 ]
Shan, Wang Xing [1 ,2 ]
Weng, Xisheng [1 ,2 ]
机构
[1] Chinese Acad Med Sci, Dept Orthoped Surg, Peking Union Med Coll Hosp, Beijing 100730, Peoples R China
[2] Peking Union Med Coll, Beijing 100730, Peoples R China
[3] Chinese Acad Med Sci, Inst Basic Med Sci, Beijing 100730, Peoples R China
基金
中国国家自然科学基金;
关键词
microRNA; glucocorticoid; mesenchymal stem cells; MESENCHYMAL STEM-CELLS; REGULATES OSTEOGENIC DIFFERENTIATION; OSTEOBLAST DIFFERENTIATION; BONE-MARROW; REDUCED EXPRESSION; DOWN-REGULATION; CANCER CELLS; MICRORNAS; PROLIFERATION; SUPPRESSES;
D O I
10.3892/ijmm.2015.2273
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
Avascular necrosis of the femoral head (ANFH) occurs following exposure to corticosteroids, and the proliferative capacity of the mesenchymal stem cells (MSCs) belonging to ANFH was reduced. The previous studies indicate that microRNA (miRNA) has an important regulatory role during proliferation and osteogenic differentiation of MSCs. Therefore, MSCs were obtained from healthy adults, and were cultured and osteogenically-induced by different dexamethasone concentrations. The proliferation and osteogenic differentiation capacities were examined through observing cellular morphology, alkaline phosphatase and alizarin red; miRNA expression was investigated using an miRNA gene chip and miRNA of differential expressions were retrieved through a database to analyze its regulatory effect. Dexamethasone at a concentration of 10(-7) mol/l induced the proliferation and osteogenic differentiation of MSCs and resulted in evident miRNA expression profile changes. In total, 11 miRNAs were upregulated at 10(-7) mol/l while 6 were downregulated, and partial miRNA was identified to participate in the regulation of cell proliferation and cell apoptosis, MSC osteogenic differentiation, lipid metabolism and other processes.
引用
收藏
页码:678 / 684
页数:7
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