Mesoporous silica hollow sphere (MSHS) for the bioelectrochemistry of horseradish peroxidase

被引：25

作者：

Cao, Zhaoxia ^{[1
,2
]}

Zhang, Jian ^{[1
,2
]}

Zeng, Julan ^{[1
,3
]}

Sun, Lixian ^{[1
]}

Xu, Fen ^{[1
]}

Cao, Zhong ^{[3
]}

Zhang, Ling ^{[3
]}

Yang, Daowu ^{[3
]}

机构：

[1] Chinese Acad Sci, Dalian Inst Chem Phys, Mat &Thermochem Lab, Dalian 116023, Liaoning Prov, Peoples R China

[2] Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China

[3] Changsha Univ Sci & Technol, Sch Chem & Environm Engn, Changsha 410076, Hunan, Peoples R China

来源：

TALANTA | 2009年 / 77卷 / 03期

基金：

中国国家自然科学基金;

关键词：

Hollow mesoporous silica spheres; Horseradish peroxidase; Biosensor; Direct electron transfer; DIRECT ELECTROCHEMISTRY; HYDROGEN-PEROXIDE; ENZYME IMMOBILIZATION; LYSOZYME ADSORPTION; CATALYTIC ACTIVITY; H2O2; BIOSENSOR; GOLD ELECTRODE; MATRIX; NANOPARTICLES; ENCAPSULATION;

D O I：

10.1016/j.talanta.2008.06.043

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

In this work, novel mesoporous silica hollow spheres (MSHS) were chosen as an immobilization matrix, to construct a mediator-free third-generation HRP biosensor. UV-vis spectroscopy revealed that horseradish peroxidase (HRP) entrapped in MSHS could retain its native Structure. FTIR spectroscopy and nitrogen adsorption-desorption isotherms indicated that HRP are intercalated into the mesopores. The direct electron transfer of HRP entrapped in MSHS was observed. A pair of stable and well-defined redox peaks of HRP with a formal potential of about -0.150V (vs. Ag/AgCl) in 0.1 M pH 7.0 phosphate-buffered solution (PBS) were obtained. The biosensor exhibited a fast amperometric response to H2O2 with a linear range of 3.9 x 10(-6) to 1.4 x 10(-4) M (R = 0.997, N = 20). The detection limit was 1.2 x 10(-6) M based S/N = 3. (C) 2008 Published by Elsevier B.V.

引用

页码：943 / 947

页数：5

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