Differential pathogenicity among Tomato leaf curl Gujarat virus isolates from India

Tomato leaf curl Gujarat virus (ToLCGV) has been identified as one of the most destructive pathogens causing tomato leaf curl disease (ToLCD) in India. In the tomato growing regions of Dhanbad and Ramgarh, plants bearing severe symptoms of ToLCD such as leaf curling, leaf crinkling, yellowing and leaf rolling was observed in the farmer fields. The association of begomovirus in these samples was confirmed by PCR and the causal viruses were identified as the isolates of ToLCGV. However, association of cognate DNA B component could not be ascertained from these samples. Indeed, like other Old World begomoviruses, the present ToLCGV isolates were found to be associated with a particular betasatellite, Tomato yellow leaf curl Thailand betasatellite (TYLCTHB). Although DNA A of both ToLCGV isolates could alone infect tomato inducing systemic symptoms, the difference in virulence was observed. Co-inoculation of TYLCTHB reduced the incubation period without influencing the accumulation of helper virus DNA and hence, differential pathogenesis among ToLCGV isolates was governed by the helper component rather than betasatellite. ToLCGV infection with DNA B increases the accumulation of DNA A component of Dhanbad isolate but not of Ramgarh isolate. Results indicated that the begomovirus identified from Ramgarh sample was a mild strain of ToLCGV.