Single immunization with a recombinant multiple-epitope protein induced protection against FMDV type Asia 1 in cattle

被引：8

作者：

Fang, Mingli ^{[1
]}

Wang, Hua ^{[1
]}

Tang, Tongyu ^{[2
]}

Zhao, Peiyan ^{[1
]}

Du, Junyang ^{[4
]}

Guo, Sheng ^{[1
]}

Wei, Hongfei ^{[1
]}

Xu, Haifei ^{[1
]}

Wan, Min ^{[1
]}

Wei, Xuefeng ^{[3
]}

Yu, Yongli ^{[4
]}

Wang, Liying ^{[1
]}

机构：

[1] Jilin Univ, Norman Bethune Coll Med, Dept Mol Biol, Changchun 130021, Peoples R China

[2] Jilin Univ, Hosp 1, Dept Gastroenterol, Changchun 130021, Peoples R China

[3] Baoling Biopharmaceut Corp, Inner Mongolia Jinyu Grp, Hohhot 013300, Inner Mongolia, Peoples R China

[4] Jilin Univ, Norman Bethune Coll Med, Dept Mol Biol Immunol, Changchun 130021, Peoples R China

来源：

INTERNATIONAL IMMUNOPHARMACOLOGY | 2015年 / 28卷 / 02期

关键词：

Foot-and-mouth disease virus (FMDV); Recombinant-epitope vaccine; Serotype Asia 1; VP1 capsid protein; MOUTH-DISEASE VIRUS; IMMUNE-RESPONSE; PEPTIDE; CELL; ANTIBODY; NEUTRALIZATION; VACCINATION; SEQUENCE; VP1; OPTIMIZATION;

D O I：

10.1016/j.intimp.2015.08.017

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

To develop recombinant epitope vaccines against the foot-and-mouth disease virus (FMDV) serotype Asia 1, genes encoding six recombinant proteins (A1-A6) consisting of different combinations of B-cell and T-cell epitopes from VP1 capsid protein (VP1) of FMDV were constructed. These proteins were expressed in Escherichia colt and used to immunize animals. Our results showed that A6 elicited higher titers of neutralizing antibodies after single inoculation in guinea pigs than did the other five recombinant proteins, as determined by microneutralization tests. In addition, a strong lymphocyte proliferation response and Th1 type immunity were observed in splenocytes from the mice immunized with A6. Further tests carried out in cattle demonstrated that a single inoculation with A6 generated comparable levels of neutralizing antibodies as inactivated vaccine and protected 4 of 5 cattle against challenge with FMDV type Asia 1. Our results suggest that A6 might be a promising recombinant vaccine against FMDV type Asia 1 in cattle. (C) 2015 Elsevier B.V. All rights reserved.

引用

页码：960 / 966

页数：7

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