Neuroprotective effect of methylprednisolone combined with placenta-derived mesenchymal stem cell in rabbit model of spinal cord injury

被引：0
作者：
Tan, Jiang-Wei [1 ]
Wang, Kai-Yan [2 ]
Liao, Guang-Jun [3 ]
Chen, Fang-Min [1 ]
Mu, Ming-Zhang [4 ]
机构：
[1] Binzhou Med Univ, Yantai Affiliated Hosp, Dept Spine Surg, Yantai 264100, Peoples R China
[2] Yantaishan Hosp, Dept Anesthesiol, Yantai 264000, Peoples R China
[3] Yantaishan Hosp, Dept Orthoped Oncol, Yantai 264000, Peoples R China
[4] Yantaishan Hosp, Dept Pediat Orthoped, Yantai 264000, Peoples R China
来源：
INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY | 2015年 / 8卷 / 08期
关键词：
Neuroprotective; methylprednisolone; placenta-derived mesenchymal stem cell; spinal cord injury; LIPID-PEROXIDATION; RATS; TESTOSTERONE; MECHANISMS; NIMODIPINE; REPAIR; TISSUE; BRAIN; TRIAL;
D O I：
暂无
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
The aim of this study was to assess the ability of the combination treatment of methylprednisolone (MP) and placenta-derived mesenchymal stem cells (PDMSCs) in a rabbit model of spinal cord injury (SCI). Rabbits were randomly divided into four groups: group 1 (control), group 2 (MP), group 3 (PDMSCs) and group 4 (MP + PDMSCs). In all groups, the spinal cord injury model was created by the weight drop method. Levels of malondialdehyde (MDA), myeloperoxidase (MPO), superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) were determined by kit. Histopathological examination was also performed. Neurological evaluation was carried out with the Tarlov scoring system. The results showed both MP and PDMSCs had neuroprotective effects, and combining the administration of MP with PDMSCs was shown a significant effect on the recovery of neurological function. Therefore, the combined use of MP and PDMSCs can be used as a potential therapeutic method for SCI.
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页码：8976 / 8982
页数：7
相关论文
共 30 条
[1]  
Atilgan M, 2012, ULUS TRAVMA ACIL CER, V18, P80, DOI [10.5505/tjtes.2011.00908, 10.5505/tjtes.2012.00908]
[2]   Methylprednisolone or tirilazad mesylate administration after acute spinal cord injury: 1-year follow up - Results of the third National Acute Spinal Cord Injury randomized controlled trial [J].
Bracken, MB ;
Shepard, MJ ;
Holford, TR ;
Leo-Summers, L ;
Aldrich, EF ;
Fazl, M ;
Fehlings, MG ;
Herr, DL ;
Hitchon, PW ;
Marshall, LF ;
Nockels, RP ;
Pascale, V ;
Perot, PL ;
Piepmeier, J ;
Sonntag, VKH ;
Wagner, F ;
Wilberger, JE ;
Winn, HR ;
Young, W .
JOURNAL OF NEUROSURGERY, 1998, 89 (05) :699-706
[3]  
Chen Z, 2011, ACTA NEUROCHIR SUPPL, V111, P289, DOI 10.1007/978-3-7091-0693-8_48
[4]   Duration of lipid peroxidation after acute spinal cord injury in rats and the effect of methylprednisolone [J].
Christie, Sean D. ;
Comeau, Ben ;
Myers, Tanya ;
Sadi, Damaso ;
Purdy, Mark ;
Mendez, Ivar .
NEUROSURGICAL FOCUS, 2008, 25 (05)
[5]   Resistant Vasospasm in Subarachnoid Hemorrhage Treated with Continuous Intraarterial Nimodipine Infusion [J].
Doukas, A. ;
Petridis, A. K. ;
Barth, H. ;
Jansen, O. ;
Maslehaty, H. ;
Mehdorn, H. M. .
TRENDS IN NEUROVASCULAR SURGERY, 2011, 112 :93-+
[6]   Acute spinal cord injury, part I: Pathophysiologic mechanisms [J].
Dumont, RJ ;
Okonkwo, DO ;
Verma, RS ;
Hurlbert, RJ ;
Boulos, PT ;
Ellegala, DB ;
Dumont, AS .
CLINICAL NEUROPHARMACOLOGY, 2001, 24 (05) :254-264
[7]   Anti-apoptotic and neuroprotective effects of alpha-lipoic acid on spinal cord ischemia-reperfusion injury in rabbits [J].
Emmez, Hakan ;
Yildirim, Zuhal ;
Kale, Aydemir ;
Tonge, Mehmet ;
Durdag, Emre ;
Borcek, Alp Ozgun ;
Ucankus, Lortlar Nese ;
Dogulu, Fikret ;
Kilic, Nedret ;
Baykaner, M. Kemali .
ACTA NEUROCHIRURGICA, 2010, 152 (09) :1591-1601
[8]   Protective effect of melatonin on experimental spinal cord ischemia [J].
Erten, SF ;
Kocak, A ;
Ozdemir, I ;
Aydemir, S ;
Colak, A ;
Reeder, BS .
SPINAL CORD, 2003, 41 (10) :533-538
[9]  
Genovese T, 2008, CURR MED CHEM, V15, P477
[10]   Neuroprotection and Acute Spinal Cord Injury: A Reappraisal [J].
Hall E.D. ;
Springer J.E. .
NeuroRX, 2004, 1 (1) :80-100
123