Preparation and characterization of high purity β-chitin from squid pens (Loligo chenisis)

被引：91

作者：

Hoang Ngoc Cuong ^{[1
]}

Nguyen Cong Minh ^{[2
]}

Nguyen Van Hoa ^{[3
]}

Trang Si Trung ^{[3
]}

机构：

[1] Binh Duong Univ, Fac Biotechnol, Thu Dau Mot, Binh Duong, Vietnam

[2] Nha Trang Univ, Inst Biotechnol & Environm, Nha Trang, Vinh Tho, Vietnam

[3] Nha Trang Univ, Fac Food Technol, Nha Trang, Vinh Tho, Vietnam

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2016年 / 93卷

关键词：

Squid pens; beta-Chitin; Loligo chenesis; CHITOSAN; DEACETYLATION; RANGE;

D O I：

10.1016/j.ijbiomac.2016.08.085

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Squid pens were extracted by a NaOH solution at 80 degrees C for 10h to remove protein and minerals. The as-prepared beta-chitin had a high molecular weight (8.5 +/- 0.1 x 10(3) kDa), a low protein content (0.63 +/- 0.02 wt.%), and a negligible amount of minerals. This method avoids the conventional method for the removal of minerals from shrimp and crab shells by HCl. The purity of resulting products was measured by NMR and FTIR. Moreover, the morphology and crystallinity of beta-chitin was characterized by SEM and XRD. The beta-chitin with long chains and high purity is suitable for producing high quantity beta-chitosan for various potential applications. (C) 2016 Elsevier B.V. All rights reserved.

引用

页码：442 / 447

页数：6

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