Expansion of PmBEAT genes in the Prunus mume genome induces characteristic floral scent production

被引：30

作者：

Bao, Fei ^{[1
]}

Ding, Anqi ^{[1
,2
]}

Zhang, Tengxun ^{[1
,2
]}

Luo, Le ^{[1
]}

Wang, Jia ^{[1
]}

Cheng, Tangren ^{[1
]}

Zhang, Qixiang ^{[1
,2
]}

机构：

[1] Beijing Forestry Univ, Beijing Key Lab Ornamental Plants Germplasm Innov, Natl Engn Res Ctr Floriculture,Beijing Lab Urban, Engn Res Ctr Landscape Environm,Minist Educ,Key L, Beijing 100083, Peoples R China

[2] Beijing Forestry Univ, Beijing Adv Innovat Ctr Tree Breeding Mol Design, Beijing 100083, Peoples R China

来源：

HORTICULTURE RESEARCH | 2019年 / 6卷

基金：

北京市自然科学基金; 中国国家自然科学基金;

关键词：

TRANSPOSABLE ELEMENTS; ACETYLTRANSFERASE; BIOSYNTHESIS; EVOLUTION; ESTERS; IDENTIFICATION; DUPLICATION; EXPRESSION; RETROGENES; ACETATE;

D O I：

10.1038/s41438-018-0104-4

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Prunus mume is the only plant in the genus Prunus of the Rosaceae family with a characteristic floral scent, and the main component of this scent is benzyl acetate. By contrast, benzyl acetate is not synthesized in Prunus persica flowers. Here, we searched for benzyl alcohol acetyltransferase (BEAT) genes based on genomic data from P. mume and P. persica and found 44 unique PmBEATs in P. mume. These genes, which were mainly detected in clusters on chromosomes, originated from gene duplication events during the species evolution of P. mume, and retroduplication and tandem duplication were the two dominant duplication patterns. The genes PmBEAT34, PmBEAT36 and PmBEAT37, which were generated by tandem duplication, were highly expressed in flowers, and their highest levels were detected during the blooming stage. In vitro, PmBEAT34, PmBEAT3, and PmBEAT37 all had benzyl alcohol acetyltransferase activity that was localized in the cytoplasm. Overexpression of the PmBEAT36 or PmBEAT37 genes increased benzyl acetate production in the petal protoplasts of P. mume, and interference in the expression of these genes slightly decreased the benzyl acetate content. In addition, light and temperature regulated the expression of the PmBEAT34, PmBEAT36 and PmBEAT37 genes. According to these results, we hypothesize that the expansion of the PmBEAT genes in the genome induce the characteristic floral scent of P. mume.

引用

页数：18

共 31 条

[31] The genome of Prunus mume [J].

Zhang, Qixiang ;

Chen, Wenbin ;

Sun, Lidan ;

Zhao, Fangying ;

Huang, Bangqing ;

Yang, Weiru ;

Tao, Ye ;

Wang, Jia ;

Yuan, Zhiqiong ;

Fan, Guangyi ;

Xing, Zhen ;

Han, Changlei ;

Pan, Huitang ;

Zhong, Xiao ;

Shi, Wenfang ;

Liang, Xinming ;

Du, Dongliang ;

Sun, Fengming ;

Xu, Zongda ;

Hao, Ruijie ;

Lv, Tian ;

Lv, Yingmin ;

Zheng, Zequn ;

Sun, Ming ;

Luo, Le ;

Cai, Ming ;

Gao, Yike ;

Wang, Junyi ;

Yin, Ye ;

Xu, Xun ;

Cheng, Tangren ;

Wang, Jun .

NATURE COMMUNICATIONS, 2012, 3

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