Genetically encoded Ca2+ indicators: Properties and evaluation

被引：129

作者：

Koldenkova, Vadim Perez ^{[1
]}

Nagai, Takeharu ^{[1
,2
]}

机构：

[1] Osaka Univ, Inst Sci & Ind Res, Dept Biomol Sci & Engn, Ibaraki, Osaka 5670047, Japan

[2] Japan Sci & Technol Agcy, PRESTO, Tokyo 1020075, Japan

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH | 2013年 / 1833卷 / 07期

关键词：

Genetically encoded calcium ion indicators; Ca2+ imaging; Fluorescent proteins; FRET; Ratiometric; Intensiometric; GREEN FLUORESCENT PROTEINS; IN-VIVO; NEURAL ACTIVITY; CALCIUM CONCENTRATIONS; PHOTOPROTEIN AEQUORIN; LIVING CELLS; TRANSIENTS; MECHANISM; DYNAMICS; SIGNALS;

D O I：

10.1016/j.bbamcr.2013.01.011

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Genetically encoded calcium ion (Ca2+) indicators have become very useful and widely used tools for Ca2+ imaging, not only in cellular models, but also in living organisms. However, the in vivo and in situ characterization of these indicators is tedious and time consuming, and it does not provide information regarding the suitability of an indicator for particular experimental environments. Thus, initial in vitro evaluation of these tools is typically performed to determine their properties. In this review, we examined the properties of dynamic range, affinity, selectivity, and kinetics for Ca2+ indicators. Commonly used strategies for evaluating these properties are presented. This article is part of a Special Issue entitled: 12th European Symposium on Calcium. (C) 2013 Elsevier B.V. All rights reserved.

引用

页码：1787 / 1797

页数：11

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