Aptamer-based label-free detection of PDGF using ruthenium(II) complex as luminescent probe

被引：21
作者：
Babu, E. [1 ]
Singaravadivel, S. [1 ]
Manojkumar, P. [2 ]
Krishnasamy, S. [2 ]
Kumar, G. Gnana [1 ]
Rajagopal, S. [1 ]
机构：
[1] Madurai Kamaraj Univ, Sch Chem, Dept Phys Chem, Madurai 625021, Tamil Nadu, India
[2] Madurai Kamaraj Univ, Sch Biotechnol, Dept Genet Engn, Madurai 625021, Tamil Nadu, India
来源：
ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2013年 / 405卷 / 21期
关键词：
Ru(II) complex; Aptamer; PDGF; Biosensor; Luminescence; GROWTH-FACTOR; DNA; BINDING;
D O I：
10.1007/s00216-013-7118-4
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
We report a simple, cost-effective, and label-free detection method, consisting of a platelet-derived growth factor (PDGF) binding aptamer and hydrophobic Ru(II) complex as a sensor system for PDGF. The binding of PDGF with the aptamer results in the weakening of the aptamer-Ru(II) complex, monitored by luminescence signal. A substantial enhancement in the luminescence intensity of Ru(II) complex is observed in the presence of aptamer due to the hydrophobic interaction. Upon addition of PDGF, the luminescence intensity is decreased, due to the stronger interaction between the aptamer and PDGF resulting in the displacement of Ru(II) complex to the aqueous solution. Our assay can detect a target specifically in a complex medium such as the mixture of proteins, at a concentration of 0.8 pM.
引用
收藏
页码：6891 / 6895
页数：5
相关论文
共 11 条
  • [1] Recognition and reaction of metallointercalators with DNA
    Erkkila, KE
    Odom, DT
    Barton, JK
    [J]. CHEMICAL REVIEWS, 1999, 99 (09) : 2777 - 2795
  • [2] Molecular aptamer for real-time oncoprotein platelet-derived growth factor monitoring by fluorescence anisotropy
    Fang, XH
    Cao, ZH
    Beck, T
    Tan, WH
    [J]. ANALYTICAL CHEMISTRY, 2001, 73 (23) : 5752 - 5757
  • [3] INTERACTION OF DELTA-[RU(PHEN)(2)DPPZ](2+) AND LAMBDA-[RU(PHEN)(2)DPPZ](2+) WITH DNA - A CALORIMETRIC AND EQUILIBRIUM BINDING STUDY
    HAQ, I
    LINCOLN, P
    SUH, DC
    NORDEN, B
    CHOWDHRY, BZ
    CHAIRES, JB
    [J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1995, 117 (17) : 4788 - 4796
  • [4] Aptamer-functionalized gold nanoparticles for turn-on light switch detection of platelet-derived growth factor
    Huang, Chih-Ching
    Chiu, Sheng-Hsien
    Huang, Yu-Fen
    Chang, Huan-Tsung
    [J]. ANALYTICAL CHEMISTRY, 2007, 79 (13) : 4798 - 4804
  • [5] Aptamer in Bioanalytical Applications
    Iliuk, Anton B.
    Hu, Lianghai
    Tao, W. Andy
    [J]. ANALYTICAL CHEMISTRY, 2011, 83 (12) : 4440 - 4452
  • [6] Signaling aptamer/protein binding by a molecular light switch complex
    Jiang, YX
    Fang, XH
    Bai, CL
    [J]. ANALYTICAL CHEMISTRY, 2004, 76 (17) : 5230 - 5235
  • [7] Absence of chiral discrimination in the interaction of tris(diphenylphenanthroline)ruthenium(II) with DNA
    Kim, HK
    Lincoln, P
    Nordén, B
    Tuite, E
    [J]. CHEMICAL COMMUNICATIONS, 1997, (24) : 2375 - 2376
  • [8] Hydroxylamine amplified gold nanoparticle-based aptameric system for the highly selective and sensitive detection of platelet-derived growth factor
    Wang, Ping
    Song, Yinhuan
    Zhao, Yanjun
    Fan, Aiping
    [J]. TALANTA, 2013, 103 : 392 - 397
  • [9] Wang RE, 2011, CURR MED CHEM, V18, P4175
  • [10] DNA-Mediated Homogeneous Binding Assays for Nucleic Acids and Proteins
    Zhang, Hongquan
    Li, Feng
    Dever, Brittany
    Li, Xing-Fang
    Le, X. Chris
    [J]. CHEMICAL REVIEWS, 2013, 113 (04) : 2812 - 2841
12