Transient pressure mediated intranuclear delivery of FITC-Dextran into chicken cardiomyocytes by MEMS-based nanothermite reaction actuator

被引：41

作者：

Korampally, Madhuri ^{[1
]}

Apperson, Steven J. ^{[1
]}

Staley, Clay S. ^{[1
,2
]}

Castorena, Jorge A. ^{[1
]}

Thiruvengadathan, Rajagopalan ^{[1
,2
]}

Gangopadhyay, Keshab ^{[1
,2
]}

Mohan, Rajiv R. ^{[1
]}

Ghosh, Arkasubhra ^{[1
]}

Polo-Parada, Luis ^{[1
]}

Gangopadhyay, Shubhra ^{[1
]}

机构：

[1] Univ Missouri, Columbia, MO 65211 USA

[2] NEMS MEMS Works LLC, Columbia, MO 65202 USA

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2012年 / 171卷

基金：

美国国家科学基金会;

关键词：

Molecular delivery; Shock wave; Nanoenergetics; Transfection; MEMS; MOLECULAR DELIVERY; DNA-TRANSFECTION; GENE DELIVERY; STRESS WAVES; EFFICIENT;

D O I：

10.1016/j.snb.2012.06.081

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

We have developed a novel, operator-friendly, nanothermite reaction actuator which generates transient pressures comprised of a shock wave superimposed on a broad pressure pulse to facilitate intranuclear molecular transport. The actuator demonstrates the delivery of similar to 70 kDa FITC-Dextran into chicken cardiomyocytes with cytoplasmic delivery efficiency greater than 90%, maximum intranuclear delivery efficiency of 84%, and cell survival rates exceeding 95% in minimum operating pressure conditions. Tunable nanothermite reactions enable versatile pressure generating characteristics which can extend the technology to a spectrum of biomedical molecular delivery applications. (C) 2012 Elsevier B.V. All rights reserved.

引用

页码：1292 / 1296

页数：5

共 18 条

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