In-vivo analysis of the uptake process of heavy metals through maize roots by using synchrotron X-ray fluorescence spectroscopy

被引:4
作者
Hwang, Bae Geun [1 ]
Lee, Sang Joon [1 ]
Gil, Kyehwan [2 ]
机构
[1] Pohang Univ Sci & Technol POSTECH, Dept Mech Engn, Pohang 37673, South Korea
[2] PAL, Pohang 37673, South Korea
基金
新加坡国家研究基金会;
关键词
XRF spectroscopy; Heavy metal; Phytoremediation; ARSENIC DISTRIBUTION; SPECTROMETRY; SPECIATION; PLANTS;
D O I
10.3938/jkps.69.1824
中图分类号
O4 [物理学];
学科分类号
0702 ;
摘要
The uptake of heavy metals by plants has been receiving much attention for crop contamination and phytoremediation. We employed synchrotron X-ray fluorescence (XRF) spectroscopy for an in-vivo analysis of heavy-metal uptake through a strand of maize root. A focused X-ray beam of 2.5 x 2.5 mu m(2) in physical dimensions was scanned along horizontal lines of the maize root at intervals of 3 mu m at the 4B X-ray micro-diffraction beamline of the Pohang Accelerator Laboratory (PAL). Time-resolved mapping of the fluorescence intensities from multiple metallic elements in the root tissues provided information about the radial distributions of heavy-metal elements and their temporal variations. The concentrated core stream of heavy-metal elements spread radially up to roughly 500 mu m, corresponding to 40 % of the root diameter. The absorption characteristics of three heavy metals, Cr, Mn and Ni, and their physiological features were analyzed. The absolute concentrations and the contents of the heavy-metal elements in the tested maize roots were quantitatively evaluated by using the calibration curve obtained from reference samples with preset concentrations. The uptake quantities of the tested heavy-metal elements are noticeably different, although their molecular weights are similar. This study should be helpful for understanding plant physiology related with heavy-metal uptake.
引用
收藏
页码:1824 / 1829
页数:6
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