Enhanced production of lignans and neolignans in chitosan-treated flax (Linum usitatissimum L.) cell cultures

被引：26

作者：

Ahmad, Waqar ^{[1
]}

Zahir, Adnan ^{[1
]}

Nadeem, Muhammad ^{[1
]}

Garros, Laurine ^{[2
,4
,5
]}

Drouet, Samantha ^{[2
,4
]}

Renouard, Sullivan ^{[2
,4
]}

Doussot, Joel ^{[2
,4
,6
]}

Giglioli-Guivarc, Nathalie ^{[3
,4
]}

Hano, Christophe ^{[2
,4
]}

Abbasi, Bilal Haider ^{[1
,2
,3
,4
]}

机构：

[1] Quaid I Azam Univ, Dept Biotechnol, Islamabad 45320, Pakistan

[2] Univ Orleans, INRA, LBLGC, Plant Lignans Team,USC1328, F-28000 Chartres, France

[3] Univ Francois Rabelais Tours, Biomol & Biotechnol Vegetales EA2106, Tours, France

[4] Univ Orleans, CNRS, GDR COSMACTIFS 3711, Bioactifs & Cosmet, Orleans, France

[5] Univ Orleans, CNRS, ICOA, UMR7311, F-45067 Orleans 2, France

[6] CNAM, CASER SITI CG, 2 Rue Conte, F-75003 Paris, France

来源：

PROCESS BIOCHEMISTRY | 2019年 / 79卷

关键词：

Linum usitatissimum; Chitosan; Lignans; Neolignans; Elicitation; Cell culture; SECONDARY METABOLITES; PHOTOPERIOD REGIMES; SUSPENSION-CULTURES; GENE-EXPRESSION; IN-VITRO; BIOSYNTHESIS; ACCUMULATION; IDENTIFICATION; PURIFICATION; STRESS;

D O I：

10.1016/j.procbio.2018.12.025

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Linum usitatissimum is a source of pharmacologically active lignans and neolignans. An effective protocol has been established for the enhanced biosynthesis of lignans and neolignans in cell cultures of Linum usitatissimum by using chitosan addition. Gene expression analysis of monolignols (PAL, CCR and CAD), lignans (DIR, PLR and UGT) and neolignans (PCBER) biosynthetic genes by RT-qPCR as well as monolignol biosynthetic PAL, CCR and CAD enzyme activities evidenced a stimulation following chitosan treatment. Validated reverse phase high-performance liquid chromatography coupled to diode array detection was used to quantify secoisolariciresinol diglucoside (SDG) and lariciresinol diglucoside (LDG), dehydrodiconiferyl alcohol glucoside (DCG) and guaiacylglycerol-beta-coniferyl alcohol ether glucoside (GGCG) showed that chitosan treated cell cultures had better accumulation of these metabolites. Maximum enhancements of 7.3-fold (28 mg/g DW) occurred for LDG, 3.5-fold (58.85 mg/g DW) in DCG and while the least enhancement of 2-fold (18.42 mg/g DW) for SDG was observed in 10 mg/l chitosan treated cell cultures than to controls. Furthermore, same concentration of chitosan also resulted in 1.3-fold increase in antioxidant activity. Compared to the lignans and neolignans accumulations observed in wild type and RNAi-PLR transgenic flaxseeds, chitosan-treated cell cultures appeared to be a very effective production system for these compounds.

引用

页码：155 / 165

页数：11

共 55 条

[1] Differential effects of in vitro cultures of Linum usitatissimum L. (Flax) on biosynthesis, stability, antibacterial and antileishmanial activities of zinc oxide nanoparticles: a mechanistic approach [J].