Long-term MRI tracking of dual-labeled adipose-derived stem cells homing into mouse carotid artery injury

被引：35
作者：
Qin, Jin-Bao [1 ,5 ]
Li, Kang-An [2 ]
Li, Xiang-Xiang [1 ,5 ]
Xie, Qing-Song [3 ]
Lin, Jia-Ying [4 ]
Ye, Kai-Chuang [1 ,5 ]
Jiang, Mi-Er [1 ,5 ]
Zhang, Gui-Xiang [2 ]
Lu, Xin-Wu [1 ,5 ]
机构：
[1] Shanghai Jiao Tong Univ, Sch Med, Shanghai Peoples Hosp Affiliated 9, Dept Vasc Surg, Shanghai 200011, Peoples R China
[2] Shanghai Jiao Tong Univ, Sch Med, Shanghai Peoples Hosp 1, Dept Radiol, Shanghai 200011, Peoples R China
[3] Cixi Municipal Peoples Hosp, Dept Neurosurg, Hangzhou, Zhejiang, Peoples R China
[4] Charite, Clin Gynecol, D-13353 Berlin, Germany
[5] Shanghai Jiao Tong Univ, Vasc Ctr, Shanghai 200011, Peoples R China
来源：
INTERNATIONAL JOURNAL OF NANOMEDICINE | 2012年 / 7卷
基金：
中国国家自然科学基金; 上海市自然科学基金;
关键词：
adipose-derived stem cells; carotid artery injury; magnetic resonance imaging; iron oxide particles; cell therapy; IRON-OXIDE NANOPARTICLES; MARROW STROMAL CELLS; GENE-THERAPY; TISSUE; DIFFERENTIATION; PROGRESSION;
D O I：
10.2147/IJN.S35647
中图分类号：
TB3 [工程材料学];
学科分类号：
0805 ; 080502 ;
摘要：
Background: Stem cell therapy has shown great promise for regenerative repair of injured or diseased tissues. Adipose-derived stem cells (ADSCs) have become increasingly attractive candidates for cellular therapy. Magnetic resonance imaging has been proven to be effective in tracking magnetic-labeled cells and evaluating their clinical relevance after cell transplantation. This study investigated the feasibility of imaging green fluorescent protein-expressing ADSCs (GFP-ADSCs) labeled with superparamagnetic iron oxide particles, and tracked them in vivo with noninvasive magnetic resonance imaging after cell transplantation in a model of mouse carotid artery injury. Methods: GFP-ADSCs were isolated from the adipose tissues of GFP mice and labeled with superparamagnetic iron oxide particles. Intracellular stability, proliferation, and viability of the labeled cells were evaluated in vitro. Next, the cells were transplanted into a mouse carotid artery injury model. Clinical 3 T magnetic resonance imaging was performed immediately before and 1, 3, 7, 14, 21, and 30 days after cell transplantation. Prussian blue staining and histological analysis were performed 7 and 30 days after transplantation. Results: GFP-ADSCs were found to be efficiently labeled with superparamagnetic iron oxide particles, with no effect on viability and proliferation. Homing of the labeled cells into the injured carotid artery tissue could be monitored by magnetic resonance imaging. Conclusion: Magnetically labeled ADSCs with expression of GFP can home into sites of vascular injury, and may provide new insights into understanding of cell-based therapy for cardiovascular lesions.
引用
收藏
页码：5191 / 5203
页数：13
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