Co-culture of cells in PDMS microsystem for sensitized artificial skin

被引：3

作者：

Griscom, L ^{[1
]}

Chateau, Y ^{[1
]}

Pennec, JP ^{[1
]}

Misery, L ^{[1
]}

Le Pioufle, B ^{[1
]}

机构：

[1] CNRS, Ecole Normale Super, BIOMIS, SATIE Lab,UMR 8029, Bruz, France

来源：

2005 3rd IEEE/EMBS Special Topic Conference on Microtechnology in Medicine and Biology | 2005年

关键词：

biomedical transducers; microsystem; nervous system;

D O I：

10.1109/MMB.2005.1548421

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

In this work a PDMS microsystem was conceived and fabricated for the development and study of an artificial skin-equivalent that includes nerve endings. A cellular coculture of keratinocytes, dorsal root ganglia neurons and spinal cord cells in microwells was developed. Microstructures were included in the microsystems, allowing infiltration of neuronal extensions to the skin-equivalent compartment. Electrophysiological measurements were conducted to measure electrical responses of the neurons in response to external stimulation of the skin.

引用

页码：184 / 187

页数：4

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